Myxoid liposarcoma with t(12;16) (q13;p11) contains site-specific differences in methylation patterns surrounding a zinc-finger gene mapped to the breakpoint region on chromosome 12

Sylvie Paulien, Claude Turc-Carel, Paola Dal Cin, Sheila Jani-Sait, Chandrika Sreekantaiah, Stanley P L Leong, Bert Vogelstein, Kenneth W. Kinzler, Avery A. Sandberg, Bert Vogelstein

Research output: Contribution to journalArticle

Abstract

The q13 to q15 region of human chromosome 12 is frequently and consistently rearranged in malignant and benign adipose tissue tumors as well as benign tumors of smooth muscle and salivary glands. A reciprocal translocation, (12;16) (q13;p11), is characteristic of the myxoid subtype of liposarcoma, whereas translocations within 12q13-14 are frequently observed in benign lipomas. We are using pulsed-field gel electrophoresis to study the 12q13-q14 region in order to detect and clone the respective translocation breakpoints in these tumors. The locus GLI, which encodes a zinc-finger protein, has been mapped to the same region as the myxoid liposarcoma breakpoint. Pulsed-field analysis of myxoid liposarcoma and lipoma DNA has allowed us to construct a 600-kilobase physical map surrounding the GLI locus, which shows that breakpoints in both types of tumor are outside this region. However, myxoid liposarcoma DNA samples contained altered restriction fragments detectable with GLI probes that were highly specific and reproducible from case to case. These altered fragments are due to highly specific and reproducible methylation differences that are unique to myxoid liposarcoma DNA. These methylation changes may prove to be useful clinically as a diagnostic tool to differentiate subtypes of liposarcoma.

Original languageEnglish (US)
Pages (from-to)7902-7907
Number of pages6
JournalCancer Research
Volume50
Issue number24
StatePublished - Dec 15 1990

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Myxoid Liposarcoma
Chromosomes, Human, Pair 12
Zinc Fingers
Methylation
Lipoma
Genes
DNA
Smooth Muscle Tumor
Liposarcoma
Neoplasms
Pulsed Field Gel Electrophoresis
Human Chromosomes
Salivary Glands
Adipose Tissue
Clone Cells
Proteins

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Myxoid liposarcoma with t(12;16) (q13;p11) contains site-specific differences in methylation patterns surrounding a zinc-finger gene mapped to the breakpoint region on chromosome 12. / Paulien, Sylvie; Turc-Carel, Claude; Cin, Paola Dal; Jani-Sait, Sheila; Sreekantaiah, Chandrika; Leong, Stanley P L; Vogelstein, Bert; Kinzler, Kenneth W.; Sandberg, Avery A.; Vogelstein, Bert.

In: Cancer Research, Vol. 50, No. 24, 15.12.1990, p. 7902-7907.

Research output: Contribution to journalArticle

Paulien, S, Turc-Carel, C, Cin, PD, Jani-Sait, S, Sreekantaiah, C, Leong, SPL, Vogelstein, B, Kinzler, KW, Sandberg, AA & Vogelstein, B 1990, 'Myxoid liposarcoma with t(12;16) (q13;p11) contains site-specific differences in methylation patterns surrounding a zinc-finger gene mapped to the breakpoint region on chromosome 12', Cancer Research, vol. 50, no. 24, pp. 7902-7907.
Paulien, Sylvie ; Turc-Carel, Claude ; Cin, Paola Dal ; Jani-Sait, Sheila ; Sreekantaiah, Chandrika ; Leong, Stanley P L ; Vogelstein, Bert ; Kinzler, Kenneth W. ; Sandberg, Avery A. ; Vogelstein, Bert. / Myxoid liposarcoma with t(12;16) (q13;p11) contains site-specific differences in methylation patterns surrounding a zinc-finger gene mapped to the breakpoint region on chromosome 12. In: Cancer Research. 1990 ; Vol. 50, No. 24. pp. 7902-7907.
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abstract = "The q13 to q15 region of human chromosome 12 is frequently and consistently rearranged in malignant and benign adipose tissue tumors as well as benign tumors of smooth muscle and salivary glands. A reciprocal translocation, (12;16) (q13;p11), is characteristic of the myxoid subtype of liposarcoma, whereas translocations within 12q13-14 are frequently observed in benign lipomas. We are using pulsed-field gel electrophoresis to study the 12q13-q14 region in order to detect and clone the respective translocation breakpoints in these tumors. The locus GLI, which encodes a zinc-finger protein, has been mapped to the same region as the myxoid liposarcoma breakpoint. Pulsed-field analysis of myxoid liposarcoma and lipoma DNA has allowed us to construct a 600-kilobase physical map surrounding the GLI locus, which shows that breakpoints in both types of tumor are outside this region. However, myxoid liposarcoma DNA samples contained altered restriction fragments detectable with GLI probes that were highly specific and reproducible from case to case. These altered fragments are due to highly specific and reproducible methylation differences that are unique to myxoid liposarcoma DNA. These methylation changes may prove to be useful clinically as a diagnostic tool to differentiate subtypes of liposarcoma.",
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