Abstract
The antioxidant action of myricetin and quercetin, the flavonoid constituents of the extract of Ginkgo biloba (EGb), on oxidative metabolism of brain neurons dissociated from the rats was examined using 2',7'-dichlorofluorescin (DCFH) which is retained within the neurons nd then oxidized by cellular hydrogen peroxide to be highly fluorescent. Incubation with myricetin or quercetin reduced the oxidation of DCFH in resting brain neurons, more profoundly than EGb. Myricetin decreased the oxidative metabolism at concentrations of 3 nM or more. It was 10 nM or more for the case of quercetin. Incubation with each flavonoid constituent also reduced the Ca2+-induced increase in the oxidative metabolism without affecting the cellular content of DCFH or the intracellular concentrations of Ca2+. Such an antioxidant action of myricetin or quercetin may be responsible for a part of the beneficial effects of EGb on brain neurons suject to ischemia.
Original language | English (US) |
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Pages (from-to) | 125-129 |
Number of pages | 5 |
Journal | Brain research |
Volume | 635 |
Issue number | 1-2 |
DOIs | |
State | Published - Jan 28 1994 |
Externally published | Yes |
Keywords
- 2′,7′-Dichlorofluorescin diacetate
- Brain neuron
- Extract of Ginkgo biloba
- Flavonoid
- Oxidative metabolism
- Reactive oxygen species
ASJC Scopus subject areas
- General Neuroscience
- Molecular Biology
- Clinical Neurology
- Developmental Biology