Myricetin and quercetin, the flavonoid constituents of Ginkgo biloba extract, greatly reduce oxidative metabolism in both resting and Ca2+-loaded brain neurons

Yasuo Oyama, Paul A. Fuchs, Norihiro Katayama, Katsuhiko Noda

Research output: Contribution to journalArticle

Abstract

The antioxidant action of myricetin and quercetin, the flavonoid constituents of the extract of Ginkgo biloba (EGb), on oxidative metabolism of brain neurons dissociated from the rats was examined using 2',7'-dichlorofluorescin (DCFH) which is retained within the neurons nd then oxidized by cellular hydrogen peroxide to be highly fluorescent. Incubation with myricetin or quercetin reduced the oxidation of DCFH in resting brain neurons, more profoundly than EGb. Myricetin decreased the oxidative metabolism at concentrations of 3 nM or more. It was 10 nM or more for the case of quercetin. Incubation with each flavonoid constituent also reduced the Ca2+-induced increase in the oxidative metabolism without affecting the cellular content of DCFH or the intracellular concentrations of Ca2+. Such an antioxidant action of myricetin or quercetin may be responsible for a part of the beneficial effects of EGb on brain neurons suject to ischemia.

Original languageEnglish (US)
Pages (from-to)125-129
Number of pages5
JournalBrain research
Volume635
Issue number1-2
DOIs
StatePublished - Jan 28 1994
Externally publishedYes

Keywords

  • 2′,7′-Dichlorofluorescin diacetate
  • Brain neuron
  • Extract of Ginkgo biloba
  • Flavonoid
  • Oxidative metabolism
  • Reactive oxygen species

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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