Myeloperoxidase gene expression in infant leukemia: A pediatric oncology group study

Carlos S. Alvarado; Garth E. Austin; Michael J. Borowitz; Jonathan J. Shuster; Andrew J. Carroll; Eric D. Austin; Muxiang Zhou; Sherif R. Zaki; Jeanette Pullen

doi:10.3109/10428199809058390

Myeloperoxidase gene expression in infant leukemia: A pediatric oncology group study

Carlos S. Alvarado, Garth E. Austin, Michael J. Borowitz, Jonathan J. Shuster, Andrew J. Carroll, Eric D. Austin, Muxiang Zhou, Sherif R. Zaki, Jeanette Pullen

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

A high incidence of co-expression of myeloid-associated antigens in infant B-precursor Acute Lymphocytic Leukemia (B-ALL) has been reported, but the significance of this finding is uncertain. To further assess myeloid differentiation and its prognostic significance in this disease, we investigated the frequency of myeloperoxidase (MPO) gene expression in the blast cells from 43 infants with B-ALL registered in a Pediatric Oncology Group (POG) Pilot Study for Treatment of Infant ALL, utilizing a molecular probe for detection of MPO messenger RNA (mRNA) by Northern blot hybridization and a monoclonal antibody to detect MPO-protein by immunohistochemical staining. Sufficient RNA for Northern blot was extracted from 32 bone marrow or blood samples. In two cases, MPO mRNA was determined by a reverse transcriptase-polymerase chain reaction assay and was negative in both cases. MPO-specific transcripts (MPO⁺) were present in 19 of 34 (56%) samples analyzed. Immunoreactive MPO protein was positive in 13 of the 20 (65%) patients studied. No correlation was found between MPO gene expression and clinical or laboratory features, karyotypic patterns or clinical outcome. The high frequency of MPO gene expression demonstrated in this study suggests that leukemogenic events in many cases of infant B-ALL appear to involve a pluripotent stem cell not yet fully committed to lymphoid differentiation.

Original language	English (US)
Pages (from-to)	145-160
Number of pages	16
Journal	Leukemia and Lymphoma
Volume	29
Issue number	1-2
DOIs	https://doi.org/10.3109/10428199809058390
State	Published - 1998
Externally published	Yes

Keywords

Infant ALL
MPO gene expression
MPO mRNA
MPO protein

ASJC Scopus subject areas

Hematology
Oncology
Cancer Research

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10.3109/10428199809058390

Cite this

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title = "Myeloperoxidase gene expression in infant leukemia: A pediatric oncology group study",

abstract = "A high incidence of co-expression of myeloid-associated antigens in infant B-precursor Acute Lymphocytic Leukemia (B-ALL) has been reported, but the significance of this finding is uncertain. To further assess myeloid differentiation and its prognostic significance in this disease, we investigated the frequency of myeloperoxidase (MPO) gene expression in the blast cells from 43 infants with B-ALL registered in a Pediatric Oncology Group (POG) Pilot Study for Treatment of Infant ALL, utilizing a molecular probe for detection of MPO messenger RNA (mRNA) by Northern blot hybridization and a monoclonal antibody to detect MPO-protein by immunohistochemical staining. Sufficient RNA for Northern blot was extracted from 32 bone marrow or blood samples. In two cases, MPO mRNA was determined by a reverse transcriptase-polymerase chain reaction assay and was negative in both cases. MPO-specific transcripts (MPO+) were present in 19 of 34 (56%) samples analyzed. Immunoreactive MPO protein was positive in 13 of the 20 (65%) patients studied. No correlation was found between MPO gene expression and clinical or laboratory features, karyotypic patterns or clinical outcome. The high frequency of MPO gene expression demonstrated in this study suggests that leukemogenic events in many cases of infant B-ALL appear to involve a pluripotent stem cell not yet fully committed to lymphoid differentiation.",

keywords = "Infant ALL, MPO gene expression, MPO mRNA, MPO protein",

author = "Alvarado, {Carlos S.} and Austin, {Garth E.} and Borowitz, {Michael J.} and Shuster, {Jonathan J.} and Carroll, {Andrew J.} and Austin, {Eric D.} and Muxiang Zhou and Zaki, {Sherif R.} and Jeanette Pullen",

note = "Funding Information: Supported in part by the following grants from the National Cancer Institute: CA-20549, CA-28476, CA-29139, CA-25408, CA-15989, CA-30969 and by a Veterans Administration Merit Award to GEA.",

year = "1998",

doi = "10.3109/10428199809058390",

language = "English (US)",

volume = "29",

pages = "145--160",

journal = "Leukemia and Lymphoma",

issn = "1042-8194",

publisher = "Informa Healthcare",

number = "1-2",

}

TY - JOUR

T1 - Myeloperoxidase gene expression in infant leukemia

T2 - A pediatric oncology group study

AU - Alvarado, Carlos S.

AU - Austin, Garth E.

AU - Borowitz, Michael J.

AU - Shuster, Jonathan J.

AU - Carroll, Andrew J.

AU - Austin, Eric D.

AU - Zhou, Muxiang

AU - Zaki, Sherif R.

AU - Pullen, Jeanette

N1 - Funding Information: Supported in part by the following grants from the National Cancer Institute: CA-20549, CA-28476, CA-29139, CA-25408, CA-15989, CA-30969 and by a Veterans Administration Merit Award to GEA.

PY - 1998

Y1 - 1998

N2 - A high incidence of co-expression of myeloid-associated antigens in infant B-precursor Acute Lymphocytic Leukemia (B-ALL) has been reported, but the significance of this finding is uncertain. To further assess myeloid differentiation and its prognostic significance in this disease, we investigated the frequency of myeloperoxidase (MPO) gene expression in the blast cells from 43 infants with B-ALL registered in a Pediatric Oncology Group (POG) Pilot Study for Treatment of Infant ALL, utilizing a molecular probe for detection of MPO messenger RNA (mRNA) by Northern blot hybridization and a monoclonal antibody to detect MPO-protein by immunohistochemical staining. Sufficient RNA for Northern blot was extracted from 32 bone marrow or blood samples. In two cases, MPO mRNA was determined by a reverse transcriptase-polymerase chain reaction assay and was negative in both cases. MPO-specific transcripts (MPO+) were present in 19 of 34 (56%) samples analyzed. Immunoreactive MPO protein was positive in 13 of the 20 (65%) patients studied. No correlation was found between MPO gene expression and clinical or laboratory features, karyotypic patterns or clinical outcome. The high frequency of MPO gene expression demonstrated in this study suggests that leukemogenic events in many cases of infant B-ALL appear to involve a pluripotent stem cell not yet fully committed to lymphoid differentiation.

AB - A high incidence of co-expression of myeloid-associated antigens in infant B-precursor Acute Lymphocytic Leukemia (B-ALL) has been reported, but the significance of this finding is uncertain. To further assess myeloid differentiation and its prognostic significance in this disease, we investigated the frequency of myeloperoxidase (MPO) gene expression in the blast cells from 43 infants with B-ALL registered in a Pediatric Oncology Group (POG) Pilot Study for Treatment of Infant ALL, utilizing a molecular probe for detection of MPO messenger RNA (mRNA) by Northern blot hybridization and a monoclonal antibody to detect MPO-protein by immunohistochemical staining. Sufficient RNA for Northern blot was extracted from 32 bone marrow or blood samples. In two cases, MPO mRNA was determined by a reverse transcriptase-polymerase chain reaction assay and was negative in both cases. MPO-specific transcripts (MPO+) were present in 19 of 34 (56%) samples analyzed. Immunoreactive MPO protein was positive in 13 of the 20 (65%) patients studied. No correlation was found between MPO gene expression and clinical or laboratory features, karyotypic patterns or clinical outcome. The high frequency of MPO gene expression demonstrated in this study suggests that leukemogenic events in many cases of infant B-ALL appear to involve a pluripotent stem cell not yet fully committed to lymphoid differentiation.

KW - Infant ALL

KW - MPO gene expression

KW - MPO mRNA

KW - MPO protein

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IS - 1-2

ER -

Myeloperoxidase gene expression in infant leukemia: A pediatric oncology group study

Abstract

Keywords

ASJC Scopus subject areas

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