Myelin subfractions in developing rat brain: characterization and sulphatide metabolism

J. A. Benjamins, K. Miller, G. M. McKhann

Research output: Contribution to journalArticle

Abstract

Centrifugation of isolated myelin on discontinuous sucrose gradients resulted in a separation into three bands and a pellet. The three bands were morphologically identical to myelin, whereas the pellet consisted primarily of vesicular membranes. These four fractions differed from one another in their lipid to protein ratios and in molar ratios of cholesterol: phospholipid:galactolipid. All of the fractions contained proteins typical of myelin, although the proportions of the proteins varied, with the pellet being the lowest in basic protein and proteolipid protein. High activity of 2,3 cyclic nucleotidase and low activity of cerebroside sulphotransferase further distinguished these fractions from the microsomal fraction. Distribution of radioactive sulphatide in the subfractions at 15 min after intracranial injection of radioactive sulphate indicated that newly labelled sulphatide first appeared in the lipid poor fractions, followed by the lipid rich fractions; results of pulse chase experiments also suggested this relationship. Several days or weeks after the injection of radioactive sulphate, most of the radioactive sulphatide was in the lipid rich fractions.

Original languageEnglish (US)
Pages (from-to)1589-1603
Number of pages15
JournalJournal of Neurochemistry
Volume20
Issue number6
StatePublished - 1973

Fingerprint

Sulfoglycosphingolipids
Myelin Sheath
Metabolism
Rats
Brain
Lipids
nucleotidase
3'-nucleotidase
galactosylceramide sulfotransferase
Sulfates
Proteins
Galactolipids
Proteolipids
Myelin Proteins
Injections
Centrifugation
Sucrose
Phospholipids
Cholesterol
Membranes

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Benjamins, J. A., Miller, K., & McKhann, G. M. (1973). Myelin subfractions in developing rat brain: characterization and sulphatide metabolism. Journal of Neurochemistry, 20(6), 1589-1603.

Myelin subfractions in developing rat brain : characterization and sulphatide metabolism. / Benjamins, J. A.; Miller, K.; McKhann, G. M.

In: Journal of Neurochemistry, Vol. 20, No. 6, 1973, p. 1589-1603.

Research output: Contribution to journalArticle

Benjamins, JA, Miller, K & McKhann, GM 1973, 'Myelin subfractions in developing rat brain: characterization and sulphatide metabolism', Journal of Neurochemistry, vol. 20, no. 6, pp. 1589-1603.
Benjamins, J. A. ; Miller, K. ; McKhann, G. M. / Myelin subfractions in developing rat brain : characterization and sulphatide metabolism. In: Journal of Neurochemistry. 1973 ; Vol. 20, No. 6. pp. 1589-1603.
@article{d7681ee5e19f4982bdce54feddc27caf,
title = "Myelin subfractions in developing rat brain: characterization and sulphatide metabolism",
abstract = "Centrifugation of isolated myelin on discontinuous sucrose gradients resulted in a separation into three bands and a pellet. The three bands were morphologically identical to myelin, whereas the pellet consisted primarily of vesicular membranes. These four fractions differed from one another in their lipid to protein ratios and in molar ratios of cholesterol: phospholipid:galactolipid. All of the fractions contained proteins typical of myelin, although the proportions of the proteins varied, with the pellet being the lowest in basic protein and proteolipid protein. High activity of 2,3 cyclic nucleotidase and low activity of cerebroside sulphotransferase further distinguished these fractions from the microsomal fraction. Distribution of radioactive sulphatide in the subfractions at 15 min after intracranial injection of radioactive sulphate indicated that newly labelled sulphatide first appeared in the lipid poor fractions, followed by the lipid rich fractions; results of pulse chase experiments also suggested this relationship. Several days or weeks after the injection of radioactive sulphate, most of the radioactive sulphatide was in the lipid rich fractions.",
author = "Benjamins, {J. A.} and K. Miller and McKhann, {G. M.}",
year = "1973",
language = "English (US)",
volume = "20",
pages = "1589--1603",
journal = "Journal of Neurochemistry",
issn = "0022-3042",
publisher = "Wiley-Blackwell",
number = "6",

}

TY - JOUR

T1 - Myelin subfractions in developing rat brain

T2 - characterization and sulphatide metabolism

AU - Benjamins, J. A.

AU - Miller, K.

AU - McKhann, G. M.

PY - 1973

Y1 - 1973

N2 - Centrifugation of isolated myelin on discontinuous sucrose gradients resulted in a separation into three bands and a pellet. The three bands were morphologically identical to myelin, whereas the pellet consisted primarily of vesicular membranes. These four fractions differed from one another in their lipid to protein ratios and in molar ratios of cholesterol: phospholipid:galactolipid. All of the fractions contained proteins typical of myelin, although the proportions of the proteins varied, with the pellet being the lowest in basic protein and proteolipid protein. High activity of 2,3 cyclic nucleotidase and low activity of cerebroside sulphotransferase further distinguished these fractions from the microsomal fraction. Distribution of radioactive sulphatide in the subfractions at 15 min after intracranial injection of radioactive sulphate indicated that newly labelled sulphatide first appeared in the lipid poor fractions, followed by the lipid rich fractions; results of pulse chase experiments also suggested this relationship. Several days or weeks after the injection of radioactive sulphate, most of the radioactive sulphatide was in the lipid rich fractions.

AB - Centrifugation of isolated myelin on discontinuous sucrose gradients resulted in a separation into three bands and a pellet. The three bands were morphologically identical to myelin, whereas the pellet consisted primarily of vesicular membranes. These four fractions differed from one another in their lipid to protein ratios and in molar ratios of cholesterol: phospholipid:galactolipid. All of the fractions contained proteins typical of myelin, although the proportions of the proteins varied, with the pellet being the lowest in basic protein and proteolipid protein. High activity of 2,3 cyclic nucleotidase and low activity of cerebroside sulphotransferase further distinguished these fractions from the microsomal fraction. Distribution of radioactive sulphatide in the subfractions at 15 min after intracranial injection of radioactive sulphate indicated that newly labelled sulphatide first appeared in the lipid poor fractions, followed by the lipid rich fractions; results of pulse chase experiments also suggested this relationship. Several days or weeks after the injection of radioactive sulphate, most of the radioactive sulphatide was in the lipid rich fractions.

UR - http://www.scopus.com/inward/record.url?scp=0015894971&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0015894971&partnerID=8YFLogxK

M3 - Article

C2 - 4352515

AN - SCOPUS:0015894971

VL - 20

SP - 1589

EP - 1603

JO - Journal of Neurochemistry

JF - Journal of Neurochemistry

SN - 0022-3042

IS - 6

ER -