TY - JOUR
T1 - MyD88 as a target of microRNA-203 in regulation of lipopolysaccharide or Bacille Calmette-Guerin induced inflammatory response of macrophage RAW264.7 cells
AU - Wei, Jun
AU - Huang, Xuelan
AU - Zhang, Zhaobo
AU - Jia, Wei
AU - Zhao, Zhijun
AU - Zhang, Ying
AU - Liu, Xiaoming
AU - Xu, Guangxian
N1 - Funding Information:
This work was supported by grants from Natural Science Foundation of China (Nos.: 31160494 , 30960275 ), the Program for New Century Excellent Talents in University (No.: NCET 11-11023 ), Postdoctoral Science Foundation of China (No.: 20110491554 ), Postdoctoral Special Science Foundation of China (No.: 2012T50834 ), and “Doctorate construction disciplines” open project of Ningxia Medical University (No.: KF2010-41 ).
PY - 2013/10
Y1 - 2013/10
N2 - MicroRNAs (miRNAs) have been demonstrated to play a pivotal role in the regulation of target gene expression at the post-transcriptional level. In order to better understand the role of microRNA-203 (miR-203) in the immunological regulation, the function of miR-203 was explored in the macrophage RAW264.7 cells against lipopolysaccharide (LPS) or Bacille Calmette-Guerin (BCG) stimulation. The results evidenced that myeloid differentiation factor 88 (MyD88) was a novel target of miR-203, miR-203 was capable of directly targeting the 3' untranslated region (3'UTR) of MyD88 and post-transcriptionally down-regulating the expression of protein. In addition, an overexpression of miR-203 in RAW264.7 cells was correlated with repressions of MyD88, as well as its downstream signaling of NF-κB (NF-κB1), TNF-α and IL-6. These results suggest that miR-203 may be an important regulator in macrophages against LPS or mycobacteria infection, which may through a mechanism of negatively regulating MyD88-dependent Toll-like receptors signaling pathway.
AB - MicroRNAs (miRNAs) have been demonstrated to play a pivotal role in the regulation of target gene expression at the post-transcriptional level. In order to better understand the role of microRNA-203 (miR-203) in the immunological regulation, the function of miR-203 was explored in the macrophage RAW264.7 cells against lipopolysaccharide (LPS) or Bacille Calmette-Guerin (BCG) stimulation. The results evidenced that myeloid differentiation factor 88 (MyD88) was a novel target of miR-203, miR-203 was capable of directly targeting the 3' untranslated region (3'UTR) of MyD88 and post-transcriptionally down-regulating the expression of protein. In addition, an overexpression of miR-203 in RAW264.7 cells was correlated with repressions of MyD88, as well as its downstream signaling of NF-κB (NF-κB1), TNF-α and IL-6. These results suggest that miR-203 may be an important regulator in macrophages against LPS or mycobacteria infection, which may through a mechanism of negatively regulating MyD88-dependent Toll-like receptors signaling pathway.
KW - Bacille Calmette-Guerin (BCG)
KW - Inflammatory response
KW - Lipopolysaccharide
KW - MiR-203
KW - Myeloid differentiation factor 88
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U2 - 10.1016/j.molimm.2013.03.004
DO - 10.1016/j.molimm.2013.03.004
M3 - Article
C2 - 23522925
AN - SCOPUS:84877635115
SN - 0161-5890
VL - 55
SP - 303
EP - 309
JO - Molecular Immunology
JF - Molecular Immunology
IS - 3-4
ER -