Mycobacterium tuberculosis resistance in pulmonary TB patients in Cameroon: A phenotypic susceptibility assay

J. Jubulis, K. Dionne, G. Osterhout, L. Ayuk, C. Awasom, P. Achu, J. Noeske, R. Warren, C. Mcarthur, N. Parrish

Research output: Contribution to journalArticle

Abstract

OBJECTIVE : To determine the resistance of Mycobacterium tuberculosis to first- And second-line agents in adult pulmonary tuberculosis (TB) patients in Cameroon using a novel phenotypic assay. SETTING : Samples were collected from TB patients at Bamenda Hospital in Bamenda, Cameroon. DESIGN : Samples were collected consecutively from adult pulmonary TB patients over a 2-month period. TREK SensititreTM MYCOTB panels were used to perform phenotypic drug susceptibility testing (DST). Susceptibility/resistance was determined by comparing minimum inhibitory concentrations to standard critical concentrations established for first- And second-line antituberculosis drugs. RESULTS : Of 103 sputum samples processed, growth on Löwenstein-Jensen media was confirmed in 78 samples, 65 of which were suitable for DST. Thirtynine strains (60%) were susceptible to all first- And second-line drugs. Five strains (8%) were categorized as multidrug-resistant TB. Two strains (3%) were classified as pre-extensively drug-resistant TB. Of those isolates susceptible to first-line drugs, 20% were resistant to at least one second-line drug. CONCLUSION: Antimicrobial resistance may be higher than assumed in TB strains in Cameroon, especially with regard to second-line drugs. There remains a need for rapid, comprehensive DST.

Original languageEnglish (US)
Pages (from-to)823-827
Number of pages5
JournalInternational Journal of Tuberculosis and Lung Disease
Volume19
Issue number7
DOIs
StatePublished - Jul 1 2015

Keywords

  • Antibiotic resistance
  • Cameroon
  • Tuberculosis

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Infectious Diseases

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