Abstract
OBJECTIVE : To determine the resistance of Mycobacterium tuberculosis to first- And second-line agents in adult pulmonary tuberculosis (TB) patients in Cameroon using a novel phenotypic assay. SETTING : Samples were collected from TB patients at Bamenda Hospital in Bamenda, Cameroon. DESIGN : Samples were collected consecutively from adult pulmonary TB patients over a 2-month period. TREK SensititreTM MYCOTB panels were used to perform phenotypic drug susceptibility testing (DST). Susceptibility/resistance was determined by comparing minimum inhibitory concentrations to standard critical concentrations established for first- And second-line antituberculosis drugs. RESULTS : Of 103 sputum samples processed, growth on Löwenstein-Jensen media was confirmed in 78 samples, 65 of which were suitable for DST. Thirtynine strains (60%) were susceptible to all first- And second-line drugs. Five strains (8%) were categorized as multidrug-resistant TB. Two strains (3%) were classified as pre-extensively drug-resistant TB. Of those isolates susceptible to first-line drugs, 20% were resistant to at least one second-line drug. CONCLUSION: Antimicrobial resistance may be higher than assumed in TB strains in Cameroon, especially with regard to second-line drugs. There remains a need for rapid, comprehensive DST.
Original language | English (US) |
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Pages (from-to) | 823-827 |
Number of pages | 5 |
Journal | International Journal of Tuberculosis and Lung Disease |
Volume | 19 |
Issue number | 7 |
DOIs | |
State | Published - Jul 1 2015 |
Keywords
- Antibiotic resistance
- Cameroon
- Tuberculosis
ASJC Scopus subject areas
- Pulmonary and Respiratory Medicine
- Infectious Diseases