Mycobacterium and aerobic actinomycete culture: Are two medium types and extended incubation times necessary?

Patricia Simner, Kelly A. Doerr, Lory K. Steinmetz, Nancy L. Wengenack

Research output: Contribution to journalArticle

Abstract

Mycobacterial cultures are historically performed using a liquid medium and a solid agar medium with an incubation period of up to 60 days. We performed a retrospective analysis of 21,494 mycobacterial and aerobic actinomycetes cultures performed over 10 months to determine whether two medium types remain necessary and to investigate whether culture incubation length can be shortened. Specimens were cultured using Bactec MGIT liquid medium and Middlebrook 7H11/S7H11 solid medium with incubation periods of 42 and 60 days, respectively. Time-to-positivity and the identity of isolates recovered from each medium were evaluated. A total of 1,205/21,494 cultures (6%) were positive on at least one medium. Of the 1,353 isolates recovered, 1,110 (82%) were nontuberculous mycobacteria, 145 (11%) were aerobic actinomycetes, and 98 (7%) were Mycobacterium tuberculosis complex. Assessing medium types, 1,121 isolates were recovered from solid medium cultures, 922 isolates were recovered from liquid medium cultures, and 690 isolates were recovered on both media. Liquid cultures were positive an average of 10 days before solid cultures when the two medium types were positive (P

Original languageEnglish (US)
Pages (from-to)1089-1093
Number of pages5
JournalJournal of Clinical Microbiology
Volume54
Issue number4
DOIs
StatePublished - Apr 1 2016
Externally publishedYes

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Actinobacteria
Mycobacterium
Culture Media
Nontuberculous Mycobacteria
Mycobacterium tuberculosis
Agar

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Mycobacterium and aerobic actinomycete culture : Are two medium types and extended incubation times necessary? / Simner, Patricia; Doerr, Kelly A.; Steinmetz, Lory K.; Wengenack, Nancy L.

In: Journal of Clinical Microbiology, Vol. 54, No. 4, 01.04.2016, p. 1089-1093.

Research output: Contribution to journalArticle

Simner, Patricia ; Doerr, Kelly A. ; Steinmetz, Lory K. ; Wengenack, Nancy L. / Mycobacterium and aerobic actinomycete culture : Are two medium types and extended incubation times necessary?. In: Journal of Clinical Microbiology. 2016 ; Vol. 54, No. 4. pp. 1089-1093.
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