MYBL2 is a potential prognostic marker that promotes cell proliferation in gallbladder cancer

Hai Bin Liang; Yang Cao; Qiang Ma; Yi Jun Shu; Zheng Wang; Fei Zhang; Yuan Yuan Ye; Huai Feng Li; Shan Shan Xiang; Xiao Ling Song; Yi Xu; Yi Chi Zhang; Run Fa Bao; Rui Yan Yuan; Yi Jian Zhang; Yun Ping Hu; Lin Jiang; Mao Lan Li; Xu An Wang; Xiang Song Wu; Wen Guang Wu; Shuai Zhao; Yong Fand; Xiao Peng Cui; Yun Shu Lu; Jian Zhou; Lei Zheng; Wei Gong; Ying Bin Liu

doi:10.1159/000475454

MYBL2 is a potential prognostic marker that promotes cell proliferation in gallbladder cancer

Hai Bin Liang, Yang Cao, Qiang Ma, Yi Jun Shu, Zheng Wang, Fei Zhang, Yuan Yuan Ye, Huai Feng Li, Shan Shan Xiang, Xiao Ling Song, Yi Xu, Yi Chi Zhang, Run Fa Bao, Rui Yan Yuan, Yi Jian Zhang, Yun Ping Hu, Lin Jiang, Mao Lan Li, Xu An Wang, Xiang Song WuWen Guang Wu, Shuai Zhao, Yong Fand, Xiao Peng Cui, Yun Shu Lu, Jian Zhou, Lei Zheng, Wei Gong, Ying Bin Liu

School of Medicine

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Background: Gallbladder cancer (GBC) is an aggressive and highly lethal biliary tract malignancy, with extremely poor prognosis. In the present study, we analyzed the potential involvement of MYBL2, a member of the Myb transcription factor family, in the carcinogenesis of human GBC. Methods: MYBL2 expression levels were measured in GBC and cholecystitis tissue specimens using quantitative real-time PCR (qRT-PCR) and immunohistochemical (IHC) assays. The effects of MYBL2 on cell proliferation and DNA synthesis were evaluated using Cell Counting Kit-8 assay (CCK-8), colony formation, and 5-ethynyl-2'-deoxyuridine (EdU) retention assay, flow cytometry analysis, western blot, and a xenograft model of GBC cells in nude mice. Results: MYBL2 expression was increased in GBC tissues and associated with histological differentiation, tumour invasion, clinical stage and unfavourable overall survival in GBC patients. The downregulation of MYBL2 expression resulted in the inhibition of GBC cell proliferation, and DNA replication in vitro, and the growth of xenografted tumours in nude mice. Conversely, MYBL2 overexpression resulted in the opposite effects. Conclusions: MYBL2 overexpression promotes GBC cell proliferation through the regulation of the cell cycle at the S and G2/M phase transitions. Thus, MYBL2 could serve as a potential prognostic and therapeutic biomarker in GBC patients.

Original language	English (US)
Pages (from-to)	2117-2131
Number of pages	15
Journal	Cellular Physiology and Biochemistry
Volume	41
Issue number	5
DOIs	https://doi.org/10.1159/000475454
State	Published - Jun 1 2017

Keywords

Cell cycle
Cell proliferation
G2/M phase
Gallbladder cancer
MYBL2
S phase

ASJC Scopus subject areas

General Medicine

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10.1159/000475454

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Liang, H. B., Cao, Y., Ma, Q., Shu, Y. J., Wang, Z., Zhang, F., Ye, Y. Y., Li, H. F., Xiang, S. S., Song, X. L., Xu, Y., Zhang, Y. C., Bao, R. F., Yuan, R. Y., Zhang, Y. J., Hu, Y. P., Jiang, L., Li, M. L., Wang, X. A., ... Liu, Y. B. (2017). MYBL2 is a potential prognostic marker that promotes cell proliferation in gallbladder cancer. Cellular Physiology and Biochemistry, 41(5), 2117-2131. https://doi.org/10.1159/000475454

Liang, HB, Cao, Y, Ma, Q, Shu, YJ, Wang, Z, Zhang, F, Ye, YY, Li, HF, Xiang, SS, Song, XL, Xu, Y, Zhang, YC, Bao, RF, Yuan, RY, Zhang, YJ, Hu, YP, Jiang, L, Li, ML, Wang, XA, Wu, XS, Wu, WG, Zhao, S, Fand, Y, Cui, XP, Lu, YS, Zhou, J, Zheng, L, Gong, W & Liu, YB 2017, 'MYBL2 is a potential prognostic marker that promotes cell proliferation in gallbladder cancer', Cellular Physiology and Biochemistry, vol. 41, no. 5, pp. 2117-2131. https://doi.org/10.1159/000475454

@article{c6847104237f45e781e1798b77b876db,

title = "MYBL2 is a potential prognostic marker that promotes cell proliferation in gallbladder cancer",

abstract = "Background: Gallbladder cancer (GBC) is an aggressive and highly lethal biliary tract malignancy, with extremely poor prognosis. In the present study, we analyzed the potential involvement of MYBL2, a member of the Myb transcription factor family, in the carcinogenesis of human GBC. Methods: MYBL2 expression levels were measured in GBC and cholecystitis tissue specimens using quantitative real-time PCR (qRT-PCR) and immunohistochemical (IHC) assays. The effects of MYBL2 on cell proliferation and DNA synthesis were evaluated using Cell Counting Kit-8 assay (CCK-8), colony formation, and 5-ethynyl-2'-deoxyuridine (EdU) retention assay, flow cytometry analysis, western blot, and a xenograft model of GBC cells in nude mice. Results: MYBL2 expression was increased in GBC tissues and associated with histological differentiation, tumour invasion, clinical stage and unfavourable overall survival in GBC patients. The downregulation of MYBL2 expression resulted in the inhibition of GBC cell proliferation, and DNA replication in vitro, and the growth of xenografted tumours in nude mice. Conversely, MYBL2 overexpression resulted in the opposite effects. Conclusions: MYBL2 overexpression promotes GBC cell proliferation through the regulation of the cell cycle at the S and G2/M phase transitions. Thus, MYBL2 could serve as a potential prognostic and therapeutic biomarker in GBC patients.",

keywords = "Cell cycle, Cell proliferation, G2/M phase, Gallbladder cancer, MYBL2, S phase",

author = "Liang, {Hai Bin} and Yang Cao and Qiang Ma and Shu, {Yi Jun} and Zheng Wang and Fei Zhang and Ye, {Yuan Yuan} and Li, {Huai Feng} and Xiang, {Shan Shan} and Song, {Xiao Ling} and Yi Xu and Zhang, {Yi Chi} and Bao, {Run Fa} and Yuan, {Rui Yan} and Zhang, {Yi Jian} and Hu, {Yun Ping} and Lin Jiang and Li, {Mao Lan} and Wang, {Xu An} and Wu, {Xiang Song} and Wu, {Wen Guang} and Shuai Zhao and Yong Fand and Cui, {Xiao Peng} and Lu, {Yun Shu} and Jian Zhou and Lei Zheng and Wei Gong and Liu, {Ying Bin}",

note = "Publisher Copyright: {\textcopyright} 2017 The Author(s)Published by S. Karger AG, Basel.",

year = "2017",

month = jun,

day = "1",

doi = "10.1159/000475454",

language = "English (US)",

volume = "41",

pages = "2117--2131",

journal = "Cellular Physiology and Biochemistry",

issn = "1015-8987",

publisher = "S. Karger AG",

number = "5",

}

TY - JOUR

T1 - MYBL2 is a potential prognostic marker that promotes cell proliferation in gallbladder cancer

AU - Liang, Hai Bin

AU - Cao, Yang

AU - Ma, Qiang

AU - Shu, Yi Jun

AU - Wang, Zheng

AU - Zhang, Fei

AU - Ye, Yuan Yuan

AU - Li, Huai Feng

AU - Xiang, Shan Shan

AU - Song, Xiao Ling

AU - Xu, Yi

AU - Zhang, Yi Chi

AU - Bao, Run Fa

AU - Yuan, Rui Yan

AU - Zhang, Yi Jian

AU - Hu, Yun Ping

AU - Jiang, Lin

AU - Li, Mao Lan

AU - Wang, Xu An

AU - Wu, Xiang Song

AU - Wu, Wen Guang

AU - Zhao, Shuai

AU - Fand, Yong

AU - Cui, Xiao Peng

AU - Lu, Yun Shu

AU - Zhou, Jian

AU - Zheng, Lei

AU - Gong, Wei

AU - Liu, Ying Bin

PY - 2017/6/1

Y1 - 2017/6/1

N2 - Background: Gallbladder cancer (GBC) is an aggressive and highly lethal biliary tract malignancy, with extremely poor prognosis. In the present study, we analyzed the potential involvement of MYBL2, a member of the Myb transcription factor family, in the carcinogenesis of human GBC. Methods: MYBL2 expression levels were measured in GBC and cholecystitis tissue specimens using quantitative real-time PCR (qRT-PCR) and immunohistochemical (IHC) assays. The effects of MYBL2 on cell proliferation and DNA synthesis were evaluated using Cell Counting Kit-8 assay (CCK-8), colony formation, and 5-ethynyl-2'-deoxyuridine (EdU) retention assay, flow cytometry analysis, western blot, and a xenograft model of GBC cells in nude mice. Results: MYBL2 expression was increased in GBC tissues and associated with histological differentiation, tumour invasion, clinical stage and unfavourable overall survival in GBC patients. The downregulation of MYBL2 expression resulted in the inhibition of GBC cell proliferation, and DNA replication in vitro, and the growth of xenografted tumours in nude mice. Conversely, MYBL2 overexpression resulted in the opposite effects. Conclusions: MYBL2 overexpression promotes GBC cell proliferation through the regulation of the cell cycle at the S and G2/M phase transitions. Thus, MYBL2 could serve as a potential prognostic and therapeutic biomarker in GBC patients.

AB - Background: Gallbladder cancer (GBC) is an aggressive and highly lethal biliary tract malignancy, with extremely poor prognosis. In the present study, we analyzed the potential involvement of MYBL2, a member of the Myb transcription factor family, in the carcinogenesis of human GBC. Methods: MYBL2 expression levels were measured in GBC and cholecystitis tissue specimens using quantitative real-time PCR (qRT-PCR) and immunohistochemical (IHC) assays. The effects of MYBL2 on cell proliferation and DNA synthesis were evaluated using Cell Counting Kit-8 assay (CCK-8), colony formation, and 5-ethynyl-2'-deoxyuridine (EdU) retention assay, flow cytometry analysis, western blot, and a xenograft model of GBC cells in nude mice. Results: MYBL2 expression was increased in GBC tissues and associated with histological differentiation, tumour invasion, clinical stage and unfavourable overall survival in GBC patients. The downregulation of MYBL2 expression resulted in the inhibition of GBC cell proliferation, and DNA replication in vitro, and the growth of xenografted tumours in nude mice. Conversely, MYBL2 overexpression resulted in the opposite effects. Conclusions: MYBL2 overexpression promotes GBC cell proliferation through the regulation of the cell cycle at the S and G2/M phase transitions. Thus, MYBL2 could serve as a potential prognostic and therapeutic biomarker in GBC patients.

KW - Cell cycle

KW - Cell proliferation

KW - G2/M phase

KW - Gallbladder cancer

KW - MYBL2

KW - S phase

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U2 - 10.1159/000475454

DO - 10.1159/000475454

M3 - Article

C2 - 28427077

AN - SCOPUS:85018479941

SN - 1015-8987

VL - 41

SP - 2117

EP - 2131

JO - Cellular Physiology and Biochemistry

JF - Cellular Physiology and Biochemistry

IS - 5

ER -

MYBL2 is a potential prognostic marker that promotes cell proliferation in gallbladder cancer

Abstract

Keywords

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