My-1, a new myeloid-specific antigen identified by a mouse monoclonal antibody

C. I. Civin, J. Mirro, M. L. Banquerigo

Research output: Contribution to journalArticle

Abstract

Antibody-secreting hybridomas were produced by fusion of P3-NS1/1Ag-4-1 mouse plasmacytoma cells with splenocytes from a mouse immunized with HL-60 human promyelocytic leukemia cells. A cloned hybridoma cell line was identified that secreted antibody against a cell surface antigen expressed on all normal peripheral blood granulocytes, on bone marrow granulocytic precursor cells, and on blast cells from 3 of 15 patients with nonlymphoid leukemia. However, the antibody did not react with normal peripheral blood lymphocytes, monocytes, platelets, or red cells, or with blast cells from 20 patients with acute lymphoblastic leukemia or from 5 patients with lymphoma. This monoclonal antibody identified a granulopoietic differentiation antigen (designated My-1) and may prove useful in the subclassification of nonlymphoid leukemia and in the investigation of hematopoiesis.

Original languageEnglish (US)
Pages (from-to)842-845
Number of pages4
JournalBlood
Volume57
Issue number5
StatePublished - 1981

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Monoclonal Antibodies
Antigens
Antibodies
Blood
Cells
Leukemia
Hybridomas
Lymphocytes
Differentiation Antigens
Surface Antigens
Platelets
Bone
Fusion reactions
Granulocyte Precursor Cells
Plasmacytoma
Hematopoiesis
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Granulocytes
Bone Marrow Cells
Monocytes

ASJC Scopus subject areas

  • Hematology

Cite this

Civin, C. I., Mirro, J., & Banquerigo, M. L. (1981). My-1, a new myeloid-specific antigen identified by a mouse monoclonal antibody. Blood, 57(5), 842-845.

My-1, a new myeloid-specific antigen identified by a mouse monoclonal antibody. / Civin, C. I.; Mirro, J.; Banquerigo, M. L.

In: Blood, Vol. 57, No. 5, 1981, p. 842-845.

Research output: Contribution to journalArticle

Civin, CI, Mirro, J & Banquerigo, ML 1981, 'My-1, a new myeloid-specific antigen identified by a mouse monoclonal antibody', Blood, vol. 57, no. 5, pp. 842-845.
Civin CI, Mirro J, Banquerigo ML. My-1, a new myeloid-specific antigen identified by a mouse monoclonal antibody. Blood. 1981;57(5):842-845.
Civin, C. I. ; Mirro, J. ; Banquerigo, M. L. / My-1, a new myeloid-specific antigen identified by a mouse monoclonal antibody. In: Blood. 1981 ; Vol. 57, No. 5. pp. 842-845.
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