Mutations in the membrane-proximal region of the influenza a virus M2 protein cytoplasmic tail have modest effects on virus replication

Shaun M. Stewart, Andrew Pekosz

Research output: Contribution to journalArticle

Abstract

Influenza A virus encodes M2, a proton channel that has been shown to be important during virus entry and assembly. In order to systematically investigate the role of the membrane-proximal residues in the M2 cytoplasmic tail in virus replication, we utilized scanning and directed alanine mutagenesis in combination with transcomplementation assays and recombinant viruses. The membrane-proximal residues 46 to 69 tolerated numerous mutations, with little, if any, effect on virus replication, suggesting that protein structure rather than individual amino acid identity in this region may be critical for M2 protein function.

Original languageEnglish (US)
Pages (from-to)12179-12187
Number of pages9
JournalJournal of Virology
Volume85
Issue number23
DOIs
StatePublished - Dec 2011

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Orthomyxoviridae
Virus Replication
virus replication
tail
mutation
Virus Assembly
Virus Internalization
viruses
Mutation
Membranes
Influenza A virus
protein structure
mutagenesis
Mutagenesis
Alanine
alanine
protons
Protons
Proteins
proteins

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Mutations in the membrane-proximal region of the influenza a virus M2 protein cytoplasmic tail have modest effects on virus replication. / Stewart, Shaun M.; Pekosz, Andrew.

In: Journal of Virology, Vol. 85, No. 23, 12.2011, p. 12179-12187.

Research output: Contribution to journalArticle

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