Mutations in the extracellular protein secretion pathway genes (eps) interfere with rugose polysaccharide production in and motility of Vibrio cholerae

Afsar Ali, Judith A. Johnson, Augusto A. Franco, Daniel J. Metzger, Terry D. Connell, J. Glenn Morris, Shanmuga Sozhamannan

Research output: Contribution to journalArticle

Abstract

Vibrio cholerae is the causal organism of the diarrheal disease cholera. The rugose variant of V. cholerae is associated with the secretion of an exopolysaccharide. The rugose polysaccharide has been shown to confer increased resistance to a variety of agents, such as chlorine, bioacids, and oxidative and osmotic stresses. It also promotes biofilm formation, thereby increasing the survival of the bacteria in the aquatic environments. Here we show that the extracellular protein secretion system (gene designated eps) is involved directly or indirectly in the production of rugose polysaccharide. A TnphoA insertion in epsD gene of the eps operon abolished the production of rugose polysaccharide, reduced the secretion of cholera toxin and hemolysin, and resulted in a nonmotile phenotype. We have constructed defined mutations of the epsD and epsE genes that affected these phenotypes and complemented these defects by plasmid clones of the respective wild-type genes. These results suggest a major role for the eps system in pathogenesis and environmental survival of V. cholerae.

Original languageEnglish (US)
Pages (from-to)1967-1974
Number of pages8
JournalInfection and Immunity
Volume68
Issue number4
DOIs
StatePublished - Apr 2000
Externally publishedYes

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Vibrio cholerae
Secretory Pathway
Polysaccharides
Mutation
Genes
Proteins
Phenotype
Hemolysin Proteins
Cholera
Cholera Toxin
Chlorine
Osmotic Pressure
Operon
Biofilms
Oxidative Stress
Plasmids
Clone Cells
Bacteria

ASJC Scopus subject areas

  • Immunology

Cite this

Mutations in the extracellular protein secretion pathway genes (eps) interfere with rugose polysaccharide production in and motility of Vibrio cholerae. / Ali, Afsar; Johnson, Judith A.; Franco, Augusto A.; Metzger, Daniel J.; Connell, Terry D.; Morris, J. Glenn; Sozhamannan, Shanmuga.

In: Infection and Immunity, Vol. 68, No. 4, 04.2000, p. 1967-1974.

Research output: Contribution to journalArticle

Ali, Afsar ; Johnson, Judith A. ; Franco, Augusto A. ; Metzger, Daniel J. ; Connell, Terry D. ; Morris, J. Glenn ; Sozhamannan, Shanmuga. / Mutations in the extracellular protein secretion pathway genes (eps) interfere with rugose polysaccharide production in and motility of Vibrio cholerae. In: Infection and Immunity. 2000 ; Vol. 68, No. 4. pp. 1967-1974.
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