Mutation of the zinc-binding metalloprotease motif affects Bacteroides fragilis toxin activity but does not affect propeptide processing

Augusto A. Franco, Simy L. Buckwold, Jai W. Shin, Miguel Ascon, Cynthia L. Sears

Research output: Contribution to journalArticlepeer-review

Abstract

To evaluate the role of the zinc-binding metalloprotease in Bacteroides fragilis toxin (BFT) processing and activity, the zinc-binding consensus sequences (H348, E349, H352, G355, H358, and M366) were mutated by site-directed-mutagenesis. Our results indicated that single point mutations in the zinc-binding metalloprotease motif do not affect BFT processing but do reduce or eliminate BFT biologic activity in vitro.

Original languageEnglish (US)
Pages (from-to)5273-5277
Number of pages5
JournalInfection and immunity
Volume73
Issue number8
DOIs
StatePublished - Aug 2005

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

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