Mutation in the peb1A locus of Campylobacter jejuni reduces interactions with epithelial cells and intestinal colonization of mice

Zhiheng Pei, Christophe Burucoa, Bernadette Grignon, Shahida Baqar, Xiao Zhe Huang, Dennis J. Kopecko, Louis Bourgeois, Jean Louis Fauchere, Martin J. Blaser

Research output: Contribution to journalArticle

Abstract

Campylobacter jejuni is one of the leading causes of bacterial diarrhea throughout the world. We previously found that PEB1 is a homolog of cluster 3 binding proteins of bacterial ABC transporters and that a C. jejuni adhesin, cell-binding factor 1 (CBF1), if not identical to, contains PEB1. A single protein migrating at approximately 27 to 28 kDa was recognized by anti-CBF1 and anti-PEB1. To determine the role that the operon encoding PEB1 plays in C. jejuni adherence, peb1A, the gene encoding PEB1, was disrupted in strain 81-176 by insertion of a kanamycin resistance gene through homologous recombination. Inactivation of this operon completely abolished expression of CBF1, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. In comparison to the wild- type strain, the mutant strain showed 50- to 100-fold less adherence to and 15-fold less invasion of epithelial cells in culture. Mouse challenge studies showed that the rate and duration of intestinal colonization by the mutant were significantly lower and shorter than with the wild-type strain. In summary, PEB1 is identical to a previously identified cell-binding factor, CBF1, in C. jejuni, and the peb1A locus plays an important role in epithelial cell interactions and in intestinal colonization in a mouse model.

Original languageEnglish (US)
Pages (from-to)938-943
Number of pages6
JournalInfection and Immunity
Volume66
Issue number3
StatePublished - Mar 1998
Externally publishedYes

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Campylobacter jejuni
Epithelial Cells
Mutation
Operon
Kanamycin Resistance
ATP-Binding Cassette Transporters
Homologous Recombination
Immunoblotting
Cell Communication
Sodium Dodecyl Sulfate
Genes
Polyacrylamide Gel Electrophoresis
Diarrhea
Carrier Proteins
Cell Culture Techniques
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Pei, Z., Burucoa, C., Grignon, B., Baqar, S., Huang, X. Z., Kopecko, D. J., ... Blaser, M. J. (1998). Mutation in the peb1A locus of Campylobacter jejuni reduces interactions with epithelial cells and intestinal colonization of mice. Infection and Immunity, 66(3), 938-943.

Mutation in the peb1A locus of Campylobacter jejuni reduces interactions with epithelial cells and intestinal colonization of mice. / Pei, Zhiheng; Burucoa, Christophe; Grignon, Bernadette; Baqar, Shahida; Huang, Xiao Zhe; Kopecko, Dennis J.; Bourgeois, Louis; Fauchere, Jean Louis; Blaser, Martin J.

In: Infection and Immunity, Vol. 66, No. 3, 03.1998, p. 938-943.

Research output: Contribution to journalArticle

Pei, Z, Burucoa, C, Grignon, B, Baqar, S, Huang, XZ, Kopecko, DJ, Bourgeois, L, Fauchere, JL & Blaser, MJ 1998, 'Mutation in the peb1A locus of Campylobacter jejuni reduces interactions with epithelial cells and intestinal colonization of mice', Infection and Immunity, vol. 66, no. 3, pp. 938-943.
Pei, Zhiheng ; Burucoa, Christophe ; Grignon, Bernadette ; Baqar, Shahida ; Huang, Xiao Zhe ; Kopecko, Dennis J. ; Bourgeois, Louis ; Fauchere, Jean Louis ; Blaser, Martin J. / Mutation in the peb1A locus of Campylobacter jejuni reduces interactions with epithelial cells and intestinal colonization of mice. In: Infection and Immunity. 1998 ; Vol. 66, No. 3. pp. 938-943.
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