Mutation affecting the 12th amino acid of the c-Ha-ras oncogene product occurs infrequently in human cancer

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Abstract

A point mutation alters the 12th amino acid of the c-Ha-ras oncogene product p21 in a human bladder cancer cell line. This is, at present, the only mutation known to result in a human transforming gene. This mutation may therefore represent a possible target for mutagenesis leading to carcinogenesis in humans. By means of restriction enzyme analysis, 29 human cancers, including 20 primary tumor tissues, derived from organs commonly exposed to environmental carcinogens, were tested for the presence of this mutation. None of ten primary bladder carcinomas exhibited the mutation; nor did nine colon carcinomas or ten carcinomas of the lung. Thus the point mutation affecting the 12th amino acid of the c-Ha-ras gene product, while a valuable model for carcinogenesis, does not appear to play a role in the development of most human epithelial cancers of the bladder, colon, or lung.

Original languageEnglish (US)
Pages (from-to)1175-1177
Number of pages3
JournalScience
Volume220
Issue number4602
StatePublished - 1983

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ras Genes
Oncogene Proteins
Amino Acids
Mutation
Carcinoma
Point Mutation
Urinary Bladder Neoplasms
Oncogene Protein p21(ras)
Neoplasms
Carcinogenesis
Environmental Carcinogens
ras Proteins
Restriction Mapping
Human Development
Oncogenes
Mutagenesis
Colonic Neoplasms
Lung Neoplasms
Colon
Urinary Bladder

ASJC Scopus subject areas

  • General

Cite this

@article{1938505915b14a5e8b1adfd8e0dc32b1,
title = "Mutation affecting the 12th amino acid of the c-Ha-ras oncogene product occurs infrequently in human cancer",
abstract = "A point mutation alters the 12th amino acid of the c-Ha-ras oncogene product p21 in a human bladder cancer cell line. This is, at present, the only mutation known to result in a human transforming gene. This mutation may therefore represent a possible target for mutagenesis leading to carcinogenesis in humans. By means of restriction enzyme analysis, 29 human cancers, including 20 primary tumor tissues, derived from organs commonly exposed to environmental carcinogens, were tested for the presence of this mutation. None of ten primary bladder carcinomas exhibited the mutation; nor did nine colon carcinomas or ten carcinomas of the lung. Thus the point mutation affecting the 12th amino acid of the c-Ha-ras gene product, while a valuable model for carcinogenesis, does not appear to play a role in the development of most human epithelial cancers of the bladder, colon, or lung.",
author = "Feinberg, {Andrew P} and Bert Vogelstein and Droller, {Michael J.} and Baylin, {Stephen B} and Nelkin, {Barry D}",
year = "1983",
language = "English (US)",
volume = "220",
pages = "1175--1177",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "4602",

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TY - JOUR

T1 - Mutation affecting the 12th amino acid of the c-Ha-ras oncogene product occurs infrequently in human cancer

AU - Feinberg, Andrew P

AU - Vogelstein, Bert

AU - Droller, Michael J.

AU - Baylin, Stephen B

AU - Nelkin, Barry D

PY - 1983

Y1 - 1983

N2 - A point mutation alters the 12th amino acid of the c-Ha-ras oncogene product p21 in a human bladder cancer cell line. This is, at present, the only mutation known to result in a human transforming gene. This mutation may therefore represent a possible target for mutagenesis leading to carcinogenesis in humans. By means of restriction enzyme analysis, 29 human cancers, including 20 primary tumor tissues, derived from organs commonly exposed to environmental carcinogens, were tested for the presence of this mutation. None of ten primary bladder carcinomas exhibited the mutation; nor did nine colon carcinomas or ten carcinomas of the lung. Thus the point mutation affecting the 12th amino acid of the c-Ha-ras gene product, while a valuable model for carcinogenesis, does not appear to play a role in the development of most human epithelial cancers of the bladder, colon, or lung.

AB - A point mutation alters the 12th amino acid of the c-Ha-ras oncogene product p21 in a human bladder cancer cell line. This is, at present, the only mutation known to result in a human transforming gene. This mutation may therefore represent a possible target for mutagenesis leading to carcinogenesis in humans. By means of restriction enzyme analysis, 29 human cancers, including 20 primary tumor tissues, derived from organs commonly exposed to environmental carcinogens, were tested for the presence of this mutation. None of ten primary bladder carcinomas exhibited the mutation; nor did nine colon carcinomas or ten carcinomas of the lung. Thus the point mutation affecting the 12th amino acid of the c-Ha-ras gene product, while a valuable model for carcinogenesis, does not appear to play a role in the development of most human epithelial cancers of the bladder, colon, or lung.

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