Mutants in trs 120 disrupt traffic from the early endosome to the late Golgi

Huaqing Cai, Yueyi Zhang, Marc Pypaert, Lee Walker, Susan Ferro-Novick

Research output: Contribution to journalArticle

Abstract

Transport protein particle (TRAPP), a large complex that mediates membrane traffic, is found in two forms (TRAPPI and -II). Both complexes share seven subunits, whereas three subunits (Trs130p, -120p, and -65p) are specific to TRAPPII. Previous studies have shown that mutations in the TRAPPII-specific gene trs130 block traffic through or from the Golgi. Surprisingly, we report that mutations in trs120 do not block general secretion. Instead, trs120 mutants accumulate aberrant membrane structures that resemble Berkeley bodies and disrupt the traffic of proteins that recycle through the early endosome. Mutants defective in recycling also display a defect in the localization of coat protein I (COPI) subunits, implying that Trs120p may participate in a COPI-dependent trafficking step on the early endosomal pathway. Furthermore, we demonstrate that Trs120p largely colocalizes with the late Golgi marker Sec7p. Our findings imply that Trs120p is required for vesicle traffic from the early endosome to the late Golgi.

Original languageEnglish (US)
Pages (from-to)823-833
Number of pages11
JournalJournal of Cell Biology
Volume171
Issue number5
DOIs
StatePublished - Dec 5 2005
Externally publishedYes

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Endosomes
Capsid Proteins
Mutation
Membranes
Protein Subunits
Recycling
Carrier Proteins
Genes
Proteins

ASJC Scopus subject areas

  • Cell Biology

Cite this

Cai, H., Zhang, Y., Pypaert, M., Walker, L., & Ferro-Novick, S. (2005). Mutants in trs 120 disrupt traffic from the early endosome to the late Golgi. Journal of Cell Biology, 171(5), 823-833. https://doi.org/10.1083/jcb.200505145

Mutants in trs 120 disrupt traffic from the early endosome to the late Golgi. / Cai, Huaqing; Zhang, Yueyi; Pypaert, Marc; Walker, Lee; Ferro-Novick, Susan.

In: Journal of Cell Biology, Vol. 171, No. 5, 05.12.2005, p. 823-833.

Research output: Contribution to journalArticle

Cai, H, Zhang, Y, Pypaert, M, Walker, L & Ferro-Novick, S 2005, 'Mutants in trs 120 disrupt traffic from the early endosome to the late Golgi', Journal of Cell Biology, vol. 171, no. 5, pp. 823-833. https://doi.org/10.1083/jcb.200505145
Cai, Huaqing ; Zhang, Yueyi ; Pypaert, Marc ; Walker, Lee ; Ferro-Novick, Susan. / Mutants in trs 120 disrupt traffic from the early endosome to the late Golgi. In: Journal of Cell Biology. 2005 ; Vol. 171, No. 5. pp. 823-833.
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