TY - JOUR
T1 - Mutant cell lines resistant to azetidine carboxylic acid
T2 - Quantitative and qualitative differences in pyrroline-5-carboxylate synthase activity
AU - Smith, R. J.
AU - Lodato, R. F.
AU - Valle, D. L.
AU - Kazakis, A.
PY - 1981/4/15
Y1 - 1981/4/15
N2 - Mutant Chinese hamster lung fibroblasts were selected that are resistant to the proline analog L-azetidine-2-carboxylic acid. Resistance in the two mutant cell lines is associated with two distinct alterations in pyrroline-5-carboxylate synthase, the enzyme that catalyzes the proline biosynthetic step leading from glutamic acid to pyrroline-5-carboxylate. In one mutant cell line, pyrroline-5-carboxylate synthase specific activity is increased 30-fold over the level in control cells. In the other mutant line, pyrroline-5-carboxylate synthase activity is not increased, but the enzyme has become insensitive to inhibition by ornithine and proline.
AB - Mutant Chinese hamster lung fibroblasts were selected that are resistant to the proline analog L-azetidine-2-carboxylic acid. Resistance in the two mutant cell lines is associated with two distinct alterations in pyrroline-5-carboxylate synthase, the enzyme that catalyzes the proline biosynthetic step leading from glutamic acid to pyrroline-5-carboxylate. In one mutant cell line, pyrroline-5-carboxylate synthase specific activity is increased 30-fold over the level in control cells. In the other mutant line, pyrroline-5-carboxylate synthase activity is not increased, but the enzyme has become insensitive to inhibition by ornithine and proline.
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U2 - 10.1016/0006-291X(81)91234-1
DO - 10.1016/0006-291X(81)91234-1
M3 - Article
C2 - 7247943
AN - SCOPUS:0019407296
SN - 0006-291X
VL - 99
SP - 789
EP - 795
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -