Mutagenicity monitoring following battlefield exposures: Molecular analysis of HPRT mutations in gulf war i veterans exposed to depleted uranium

Janice A. Nicklas, Richard J. Albertini, Pamela M. Vacek, Stephanie K. Ardell, Elizabeth W. Carter, Melissa A. McDiarmid, Susan M. Engelhardt, Patricia W. Gucer, Katherine S. Squibb

Research output: Contribution to journalArticlepeer-review

Abstract

Molecular studies that involved cDNA and genomic DNA sequencing as well as multiplex PCR of the HPRT gene were performed to determine the molecular mutational spectrum for 1,377 HPRT mutant isolates obtained from 61 Veterans of the 1991 Gulf War, most of whom were exposed to depleted uranium (DU). Mutant colonies were isolated from one to four times from each Veteran (in 2003, 2005, 2007, and/or 2009). The relative frequencies of the various types of mutations (point mutations, deletions, insertions, etc.) were compared between high versus low DU exposed groups, (based on their urine U concentration levels), with HPRT mutant frequency (as determined in the companion paper) and with a database of historic controls. The mutational spectrum includes all classes of gene mutations with no significant differences observed in Veterans related to their DU exposures.

Original languageEnglish (US)
Pages (from-to)594-608
Number of pages15
JournalEnvironmental and Molecular Mutagenesis
Volume56
Issue number7
DOIs
StatePublished - Aug 1 2015
Externally publishedYes

Keywords

  • Depleted uranium
  • DNA sequencing
  • HPRT
  • HPRT mutations

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis
  • Epidemiology
  • Genetics(clinical)
  • Medicine(all)

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