TY - JOUR
T1 - Mutagenicity monitoring following battlefield exposures
T2 - Molecular analysis of HPRT mutations in gulf war i veterans exposed to depleted uranium
AU - Nicklas, Janice A.
AU - Albertini, Richard J.
AU - Vacek, Pamela M.
AU - Ardell, Stephanie K.
AU - Carter, Elizabeth W.
AU - McDiarmid, Melissa A.
AU - Engelhardt, Susan M.
AU - Gucer, Patricia W.
AU - Squibb, Katherine S.
PY - 2015/8/1
Y1 - 2015/8/1
N2 - Molecular studies that involved cDNA and genomic DNA sequencing as well as multiplex PCR of the HPRT gene were performed to determine the molecular mutational spectrum for 1,377 HPRT mutant isolates obtained from 61 Veterans of the 1991 Gulf War, most of whom were exposed to depleted uranium (DU). Mutant colonies were isolated from one to four times from each Veteran (in 2003, 2005, 2007, and/or 2009). The relative frequencies of the various types of mutations (point mutations, deletions, insertions, etc.) were compared between high versus low DU exposed groups, (based on their urine U concentration levels), with HPRT mutant frequency (as determined in the companion paper) and with a database of historic controls. The mutational spectrum includes all classes of gene mutations with no significant differences observed in Veterans related to their DU exposures.
AB - Molecular studies that involved cDNA and genomic DNA sequencing as well as multiplex PCR of the HPRT gene were performed to determine the molecular mutational spectrum for 1,377 HPRT mutant isolates obtained from 61 Veterans of the 1991 Gulf War, most of whom were exposed to depleted uranium (DU). Mutant colonies were isolated from one to four times from each Veteran (in 2003, 2005, 2007, and/or 2009). The relative frequencies of the various types of mutations (point mutations, deletions, insertions, etc.) were compared between high versus low DU exposed groups, (based on their urine U concentration levels), with HPRT mutant frequency (as determined in the companion paper) and with a database of historic controls. The mutational spectrum includes all classes of gene mutations with no significant differences observed in Veterans related to their DU exposures.
KW - Depleted uranium
KW - DNA sequencing
KW - HPRT
KW - HPRT mutations
UR - http://www.scopus.com/inward/record.url?scp=84938745268&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84938745268&partnerID=8YFLogxK
U2 - 10.1002/em.21956
DO - 10.1002/em.21956
M3 - Article
C2 - 25914382
AN - SCOPUS:84938745268
SN - 0893-6692
VL - 56
SP - 594
EP - 608
JO - Environmental and Molecular Mutagenesis
JF - Environmental and Molecular Mutagenesis
IS - 7
ER -