Mutagenesis and analysis of mold Aspergillus niger for extracellular glucose oxidase production using sugarcane molasses

O. V. Singh

Research output: Contribution to journalArticlepeer-review

Abstract

Aspergillus niger ORS-4.410, a mutant of A. niger ORS-4, was generated by repeated ultraviolet (UV) irradiation. Analysis of the UV treatment dose on wild-type (WT) A. niger ORS-4, conidial survival, and frequency of mutation showed that the maximum frequency of positive mutants (25.5%) was obtained with a 57% conidial survival rate after the second stage of UV irradiation. The level of glucose oxidase (GOX) production from mutant A. niger ORS-4.410 thus obtained was 149% higher than that for WT strain A. niger ORS-4 under liquid culture conditions using hexacyanoferrate (HCF)-treated sugarcane molasses (TM) as a cheaper carbohydrate source. When subcultured monthly for 24 mo, the mutant strain had consistent levels of GOX production (2.62 ± 0.51 U/mL). Mutant A. niger ORS-4.410 was markedly different from the parent strain morphologically and was found to grow abundantly on sugarcane molasses. The mutant strain showed 3.43-fold increases in GOX levels (2.62 ± 0.51 U/mL) using HCF-TM compared with the crude form of cane molasses (0.762 ± 0.158 U/mL).

Original languageEnglish (US)
Pages (from-to)43-57
Number of pages15
JournalApplied Biochemistry and Biotechnology
Volume135
Issue number1
DOIs
StatePublished - Oct 2006

Keywords

  • Aspergillus niger
  • Glucose oxidase
  • Mutation
  • Submerged fermentation
  • Sugarcane molasses

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology

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