Abstract
Aspergillus niger ORS-4.410, a mutant of A. niger ORS-4, was generated by repeated ultraviolet (UV) irradiation. Analysis of the UV treatment dose on wild-type (WT) A. niger ORS-4, conidial survival, and frequency of mutation showed that the maximum frequency of positive mutants (25.5%) was obtained with a 57% conidial survival rate after the second stage of UV irradiation. The level of glucose oxidase (GOX) production from mutant A. niger ORS-4.410 thus obtained was 149% higher than that for WT strain A. niger ORS-4 under liquid culture conditions using hexacyanoferrate (HCF)-treated sugarcane molasses (TM) as a cheaper carbohydrate source. When subcultured monthly for 24 mo, the mutant strain had consistent levels of GOX production (2.62 ± 0.51 U/mL). Mutant A. niger ORS-4.410 was markedly different from the parent strain morphologically and was found to grow abundantly on sugarcane molasses. The mutant strain showed 3.43-fold increases in GOX levels (2.62 ± 0.51 U/mL) using HCF-TM compared with the crude form of cane molasses (0.762 ± 0.158 U/mL).
Original language | English (US) |
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Pages (from-to) | 43-57 |
Number of pages | 15 |
Journal | Applied Biochemistry and Biotechnology |
Volume | 135 |
Issue number | 1 |
DOIs | |
State | Published - Oct 2006 |
Keywords
- Aspergillus niger
- Glucose oxidase
- Mutation
- Submerged fermentation
- Sugarcane molasses
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Biochemistry
- Applied Microbiology and Biotechnology
- Molecular Biology