Muscarinic cholinergic binding in rat brain

H. I. Yamamura, Solomon H Snyder

Research output: Contribution to journalArticle

Abstract

Binding sites with high affinity and specificity for [3H]quinuclidinyl benzilate (QNB) are present in homogenates of rat brain. The characteristics of the binding sites resemble those of muscarinic cholinergic receptors. Specific binding is saturable with respect to [3H]QNB and tissue concentration and is time, temperature, and pH dependent. The bimolecular rate of association (2.0 x 108 M-1 min-1) and dissociation (1.2 x 10-2 min-1) at 35°C indicate a dissociation constant of 60 pM and a density of 65 pmol/g of brain. Muscarinic antagonists and agonists displace specific [3H]QNB binding, while nicotinic and noncholinergic drugs possess little affinity for [3H]QNB binding sites.

Original languageEnglish (US)
Pages (from-to)1725-1729
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume71
Issue number5
StatePublished - 1974

Fingerprint

Quinuclidinyl Benzilate
Cholinergic Agents
Brain
Binding Sites
Muscarinic Agonists
Muscarinic Antagonists
Cholinergic Receptors
Muscarinic Receptors
Temperature
Pharmaceutical Preparations

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Muscarinic cholinergic binding in rat brain. / Yamamura, H. I.; Snyder, Solomon H.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 71, No. 5, 1974, p. 1725-1729.

Research output: Contribution to journalArticle

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