TY - JOUR
T1 - Murine skin and vaginal mucosa are similarly susceptible to infection by pseudovirions of different papillomavirus classifications and species
AU - Handisurya, Alessandra
AU - Day, Patricia M.
AU - Thompson, Cynthia D.
AU - Buck, Christopher B.
AU - Kwak, Kihyuck
AU - Roden, Richard B.S.
AU - Lowy, Douglas R.
AU - Schiller, John T.
N1 - Funding Information:
This research was supported by the Intramural Research Program of the National Institutes of Health, National Cancer Institute, Center for Cancer Research and the Austrian Science Fund FWF (Erwin Schroedinger Fellowship project no.: J3012-B13 to AH) and Public Health Service grants CA133749 , CA118790 and P50 CA098252 .
PY - 2012/11/25
Y1 - 2012/11/25
N2 - Depending upon viral genotype, productive papillomavirus infection and disease display preferential tropism for cutaneous or mucosal stratified squamous epithelia, although the mechanisms are unclear. To investigate papillomavirus entry tropism, we used reporter pseudovirions based on various cutaneous and mucosal papillomavirus species, including the recently identified murine papillomavirus. Pseudovirus transduction of BALB/c mice was examined using an improved murine skin infection protocol and a previously developed cervicovaginal challenge model. In the skin, HPV5, HPV6, HPV16, BPV1 and MusPV1 pseudovirions preferentially transduced keratinocytes at sites of trauma, similar to the genital tract. Skin infection, visualized by in vivo imaging using a luciferase reporter gene, peaked between days 2-3 and rapidly diminished for all pseudovirion types. Murine cutaneous and genital tissues were similarily permissive for pseudovirions of HPV types 5, 6, 8, 16, 18, 26, 44, 45, 51, 58 and animal papillomaviruses BPV1 and MusPV1, implying that papillomavirus' tissue and host tropism is governed primarily by post-entry regulatory events in the mouse.
AB - Depending upon viral genotype, productive papillomavirus infection and disease display preferential tropism for cutaneous or mucosal stratified squamous epithelia, although the mechanisms are unclear. To investigate papillomavirus entry tropism, we used reporter pseudovirions based on various cutaneous and mucosal papillomavirus species, including the recently identified murine papillomavirus. Pseudovirus transduction of BALB/c mice was examined using an improved murine skin infection protocol and a previously developed cervicovaginal challenge model. In the skin, HPV5, HPV6, HPV16, BPV1 and MusPV1 pseudovirions preferentially transduced keratinocytes at sites of trauma, similar to the genital tract. Skin infection, visualized by in vivo imaging using a luciferase reporter gene, peaked between days 2-3 and rapidly diminished for all pseudovirion types. Murine cutaneous and genital tissues were similarily permissive for pseudovirions of HPV types 5, 6, 8, 16, 18, 26, 44, 45, 51, 58 and animal papillomaviruses BPV1 and MusPV1, implying that papillomavirus' tissue and host tropism is governed primarily by post-entry regulatory events in the mouse.
KW - In vivo imaging
KW - Murine model
KW - Murine papillomavirus
KW - Papillomavirus
KW - Pseudovirions
KW - Skin infection
KW - Tissue tropism
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U2 - 10.1016/j.virol.2012.08.035
DO - 10.1016/j.virol.2012.08.035
M3 - Article
C2 - 22985477
AN - SCOPUS:84867206591
SN - 0042-6822
VL - 433
SP - 385
EP - 394
JO - Virology
JF - Virology
IS - 2
ER -