TY - JOUR
T1 - Murine model of surgically induced acute aortic dissection type A
AU - Matt, Peter
AU - Huso, David L.
AU - Habashi, Jennifer
AU - Holm, Tammy
AU - Doyle, Jeff
AU - Schoenhoff, Florian
AU - Liu, Guosheng
AU - Black, James
AU - Van Eyk, Jennifer E.
AU - Dietz, Harry C.
N1 - Copyright:
Copyright 2010 Elsevier B.V., All rights reserved.
PY - 2010/4
Y1 - 2010/4
N2 - Objectives: This study aimed at developing a murine model of surgically induced acute aortic dissection type A for investigation of the formation and progression of acute aortic dissection and to test whether this system could be used for biomarker discovery. Methods: Adult fibrillin-1 deficient, Fbn1C1039G/+ mice and wild-type mice were anesthetized, ventilated, and the ascending aorta exposed via hemisternotomy. We hypothesized that acute aortic dissectiion could be induced either by injecting autologous blood into the aortic wall or by injury to the wall with aortic clamping. Echocardiography was done preoperatively, and serum samples were collected before and 30 minutes after the operation and analyzed by enzyme-linked immunosorbent assay. Results: Echocardiography revealed larger aortic root diameters in Fbn1C1039G/+ compared with wild-type mice (P = .001). Histologic examination showed that aortic clamp injury but not injection of blood leads to large intimal tears, disruption of aortic wall structures, and localized dissection of the aortic media in Fbn1C1039G/+ mice. Acute aortic dissection developed in 4 of 5 Fbn1C1039G/+ mice versus 0 of 5 wild-type mice after aortic clamping (P < .01). Elastin staining showed higher elastic fiber fragmentation and disarray in Fbn1C1039G/+ compared with wild-type mice. Enzyme-linked immunosorbent assay analysis revealed elevated circulating transforming growth factor β1 concentrations after induction of acute aortic dissection in Fbn1C1039G/+ mice (P = .02, 150 ± 61 ng/mL vs 456 ± 97 ng/mL), but not in wild-type or sham-operated mice. Conclusions: Aortic clamp injury can induce AAD in Fbn1C1039G/+, but not in wild-type mice. This murine model of surgically induced acute aortic dissection is highly reproducible and nonlethal in the short term. Using this system, we revealed that circulating transforming growth factor β1 is a promising biomarker for acute aortic dissection.
AB - Objectives: This study aimed at developing a murine model of surgically induced acute aortic dissection type A for investigation of the formation and progression of acute aortic dissection and to test whether this system could be used for biomarker discovery. Methods: Adult fibrillin-1 deficient, Fbn1C1039G/+ mice and wild-type mice were anesthetized, ventilated, and the ascending aorta exposed via hemisternotomy. We hypothesized that acute aortic dissectiion could be induced either by injecting autologous blood into the aortic wall or by injury to the wall with aortic clamping. Echocardiography was done preoperatively, and serum samples were collected before and 30 minutes after the operation and analyzed by enzyme-linked immunosorbent assay. Results: Echocardiography revealed larger aortic root diameters in Fbn1C1039G/+ compared with wild-type mice (P = .001). Histologic examination showed that aortic clamp injury but not injection of blood leads to large intimal tears, disruption of aortic wall structures, and localized dissection of the aortic media in Fbn1C1039G/+ mice. Acute aortic dissection developed in 4 of 5 Fbn1C1039G/+ mice versus 0 of 5 wild-type mice after aortic clamping (P < .01). Elastin staining showed higher elastic fiber fragmentation and disarray in Fbn1C1039G/+ compared with wild-type mice. Enzyme-linked immunosorbent assay analysis revealed elevated circulating transforming growth factor β1 concentrations after induction of acute aortic dissection in Fbn1C1039G/+ mice (P = .02, 150 ± 61 ng/mL vs 456 ± 97 ng/mL), but not in wild-type or sham-operated mice. Conclusions: Aortic clamp injury can induce AAD in Fbn1C1039G/+, but not in wild-type mice. This murine model of surgically induced acute aortic dissection is highly reproducible and nonlethal in the short term. Using this system, we revealed that circulating transforming growth factor β1 is a promising biomarker for acute aortic dissection.
UR - http://www.scopus.com/inward/record.url?scp=77949487264&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77949487264&partnerID=8YFLogxK
U2 - 10.1016/j.jtcvs.2009.08.039
DO - 10.1016/j.jtcvs.2009.08.039
M3 - Article
C2 - 19910001
AN - SCOPUS:77949487264
VL - 139
SP - 1041
EP - 1047
JO - Journal of Thoracic and Cardiovascular Surgery
JF - Journal of Thoracic and Cardiovascular Surgery
SN - 0022-5223
IS - 4
ER -