Murine intestinal intraepithelial lymphocytes I. Relationship of a novel Thy-1-, Lyt-1-, Lyt-2+, granulated subpopulation to natural killer cells and mast cells

A. Petit, P. B. Ernst, A. D. Befus, D. A. Clark, K. L. Rosenthal, T. Ishizaka, J. Bienenstock

Research output: Contribution to journalArticle

Abstract

Highly purified populations of intraepithelial lymphocytes (IEL) were obtained from the murine small intestine. We found that 84% of IEL expressed Lyt-2 and that 45-55% possessed the unique phenotype, Thy-1-, Lyt-1-, Lyt-2+. Sixty percent of IEL had granules in their cytoplasm and thus resembled the large granulated lymphocytes associated with natural killer (NK) activity. However, less that 15% of IEL had NK activity in 6-h assay. This activity resided in a subpopulation of Lyt-2- lymphocytes, leaving a large population of Thy-1-, Lyt-1-, Lyt-2+ granulated cells (45-55% of IEL) with unknown function. Sensitive radioenzymic assays for histamine showed that IEL from healthy CBA mice do not contain this amine. IgE-binding assays revealed that IEL, unlike mast cells, do not carry high-affinity receptors for IgE. Thus, only a small percentage of granulated IEL (-, Lyt-1-, Lyt-2+ and have unknown function.

Original languageEnglish (US)
Pages (from-to)211-215
Number of pages5
JournalEuropean Journal of Immunology
Volume15
Issue number3
DOIs
StatePublished - 1985

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Mast Cells
Natural Killer Cells
Lymphocytes
IgE Receptors
Inbred CBA Mouse
Immunoglobulin E
Histamine
Population
Small Intestine
Amines
Cytoplasm
Phenotype

ASJC Scopus subject areas

  • Immunology

Cite this

Murine intestinal intraepithelial lymphocytes I. Relationship of a novel Thy-1-, Lyt-1-, Lyt-2+, granulated subpopulation to natural killer cells and mast cells. / Petit, A.; Ernst, P. B.; Befus, A. D.; Clark, D. A.; Rosenthal, K. L.; Ishizaka, T.; Bienenstock, J.

In: European Journal of Immunology, Vol. 15, No. 3, 1985, p. 211-215.

Research output: Contribution to journalArticle

Petit, A. ; Ernst, P. B. ; Befus, A. D. ; Clark, D. A. ; Rosenthal, K. L. ; Ishizaka, T. ; Bienenstock, J. / Murine intestinal intraepithelial lymphocytes I. Relationship of a novel Thy-1-, Lyt-1-, Lyt-2+, granulated subpopulation to natural killer cells and mast cells. In: European Journal of Immunology. 1985 ; Vol. 15, No. 3. pp. 211-215.
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AU - Befus, A. D.

AU - Clark, D. A.

AU - Rosenthal, K. L.

AU - Ishizaka, T.

AU - Bienenstock, J.

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