Murine full-thickness skin transplantation

Chih Hsien Cheng, Chen Fang Lee, Madeline Fryer, Georg J. Furtmüller, Byoungchol Oh, Jonathan D. Powell, Gerald Brandacher

Research output: Research - peer-reviewArticle

Abstract

Murine full-thickness skin transplantation is a well-established in vivo model to study alloimmune response and graft rejection. Despite its limited application to humans, skin transplantation in mice has been widely employed for transplantation research. The procedure is easy to learn and perform, and it does not require delicate microsurgical techniques nor extensive training. Moreover, graft rejection in this model occurs in a very reproducible immunological reaction and is easily monitored by direct inspection and palpation. In addition, secondary skin transplantation with donor-matched or third-party skin grafts can be performed on more complex transplant models as an alternative and uncomplicated method to assess donor-specific tolerance. The complications are low and are in general limited to anesthesia overdose or respiratory distress after the procedure. Graft failure, on the other hand, occurs commonly as a result of poor preparation of the graft, incorrect positioning in the graft bed, or inappropriate placement of the bandage. In this article, we present a protocol for full-thickness skin transplantation in mice and describe the important steps necessary for a successful procedure.

LanguageEnglish (US)
Article numbere55105
JournalJournal of Visualized Experiments
Volume2017
Issue number119
DOIs
StatePublished - Jan 2 2017

Fingerprint

Skin Transplantation
Transplants
Grafts
Skin
Graft Rejection
Palpation
Bandages
Anesthesia
Transplantation
Research
Transplantation (surgical)
Inspection

Keywords

  • Alloimmune
  • Full-thickness skin transplantation
  • Immunology
  • In vivo
  • Issue 119
  • Metabolism
  • Mouse model
  • Rejection

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Cheng, C. H., Lee, C. F., Fryer, M., Furtmüller, G. J., Oh, B., Powell, J. D., & Brandacher, G. (2017). Murine full-thickness skin transplantation. Journal of Visualized Experiments, 2017(119), [e55105]. DOI: 10.3791/55105

Murine full-thickness skin transplantation. / Cheng, Chih Hsien; Lee, Chen Fang; Fryer, Madeline; Furtmüller, Georg J.; Oh, Byoungchol; Powell, Jonathan D.; Brandacher, Gerald.

In: Journal of Visualized Experiments, Vol. 2017, No. 119, e55105, 02.01.2017.

Research output: Research - peer-reviewArticle

Cheng, CH, Lee, CF, Fryer, M, Furtmüller, GJ, Oh, B, Powell, JD & Brandacher, G 2017, 'Murine full-thickness skin transplantation' Journal of Visualized Experiments, vol 2017, no. 119, e55105. DOI: 10.3791/55105
Cheng CH, Lee CF, Fryer M, Furtmüller GJ, Oh B, Powell JD et al. Murine full-thickness skin transplantation. Journal of Visualized Experiments. 2017 Jan 2;2017(119). e55105. Available from, DOI: 10.3791/55105
Cheng, Chih Hsien ; Lee, Chen Fang ; Fryer, Madeline ; Furtmüller, Georg J. ; Oh, Byoungchol ; Powell, Jonathan D. ; Brandacher, Gerald. / Murine full-thickness skin transplantation. In: Journal of Visualized Experiments. 2017 ; Vol. 2017, No. 119.
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