Murine epidermal Vγ5/Vδ1-T-cell receptor+ T cells respond to B-cell lines and lipolpolysaccharides

C. L. Reardon, K. Heyborne, M. Tsuji, Fidel P Zavala, R. E. Tigelaar, R. L. O'Brien, W. K. Born

Research output: Contribution to journalArticle

Abstract

The Vγ5/Vδ1+-T-cell receptor (TCR)-bearing T-cell clone, 2CBET-3, was generated from C57BL/6 mice. Upon stimulation, 2CBET-3 cells produce interleukin (IL)-3, granulocyte-macrophage colony-stimulating factor, and tumor necrosis factor-α, but not IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, macrophage colony-stimulating factor, or interferon-γ. These cells were evaluated for their ability to be stimulated by a variety of murine cell lines, including fibroblasts, trophoblasts, melanoma cells, embryonic carcinomas, B-cell lymphomas, mastocytoma cells, and keratinocytes. The human B-lymphoma cell line, Daudi, also was included in these studies. We found that 2CBET-3 cells produced cytokines up to several hundredfold above the control levels in response to the B-cell lines, Daudi, and A20/2J, but not to the B-cell line 439.4.2. After fixation with glutaraldehyde, Daudi and A20/2J continued to stimulate this γδ T-cell line. 2CBET-3 cells also responded to the keratinocyte line PAM212, but not to another, XB-2. When lipopolysaccharides (LPS) from Escherichia coli or S. typhimurium were added to 2CBET-3 cells in the presence of A20/2J cells, 2CBET-3 cells responded with increased cytokine production compared with the cytokine production in the presence of A20/2J cells alone. 2CBET-3 cells by themselves did not respond to LPS alone or to supernatants from A20/2J cells incubated with LPS. Unlike 2CBET-3, the epidermal T-cell hybridoma 70BET-49, expressing a Vγ5/Vδ1-TCR identical to that of 2CBET-3, did not respond to A20/2J cells in the presence or absence of LPS, suggesting a requirement for molecules other than the TCR for Vγ5/Vδ1-TCR+ T-cell stimulation by the B-cell lines and by LPS. This unique reactivity of γδ-TCR+ cells is different from that of αβ-TCR+ cells and may reflect a functional specialization of γδTCR+ cells in the response to bacterial infections.

Original languageEnglish (US)
JournalJournal of Investigative Dermatology
Volume105
Issue number1 SUPPL.
StatePublished - 1995
Externally publishedYes

Fingerprint

T-cells
T-Cell Antigen Receptor
B-Lymphocytes
Cells
T-Lymphocytes
Cell Line
Lipopolysaccharides
Cytokines
Bearings (structural)
Macrophage Colony-Stimulating Factor
Interleukin-3
Level control
Interleukin-5
Glutaral
Fibroblasts
Granulocyte-Macrophage Colony-Stimulating Factor
Interleukin-8
Interleukin-4
Interleukin-10
Escherichia coli

Keywords

  • B lymphocyte
  • T lymphocyte

ASJC Scopus subject areas

  • Dermatology

Cite this

Reardon, C. L., Heyborne, K., Tsuji, M., Zavala, F. P., Tigelaar, R. E., O'Brien, R. L., & Born, W. K. (1995). Murine epidermal Vγ5/Vδ1-T-cell receptor+ T cells respond to B-cell lines and lipolpolysaccharides. Journal of Investigative Dermatology, 105(1 SUPPL.).

Murine epidermal Vγ5/Vδ1-T-cell receptor+ T cells respond to B-cell lines and lipolpolysaccharides. / Reardon, C. L.; Heyborne, K.; Tsuji, M.; Zavala, Fidel P; Tigelaar, R. E.; O'Brien, R. L.; Born, W. K.

In: Journal of Investigative Dermatology, Vol. 105, No. 1 SUPPL., 1995.

Research output: Contribution to journalArticle

Reardon, CL, Heyborne, K, Tsuji, M, Zavala, FP, Tigelaar, RE, O'Brien, RL & Born, WK 1995, 'Murine epidermal Vγ5/Vδ1-T-cell receptor+ T cells respond to B-cell lines and lipolpolysaccharides', Journal of Investigative Dermatology, vol. 105, no. 1 SUPPL..
Reardon, C. L. ; Heyborne, K. ; Tsuji, M. ; Zavala, Fidel P ; Tigelaar, R. E. ; O'Brien, R. L. ; Born, W. K. / Murine epidermal Vγ5/Vδ1-T-cell receptor+ T cells respond to B-cell lines and lipolpolysaccharides. In: Journal of Investigative Dermatology. 1995 ; Vol. 105, No. 1 SUPPL.
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