Murine epidermal Langerhans cells and splenic dendritic cells present tumor-associated antigens to primed T cells

Philip J. Cohen, Peter A. Cohen, Steven A. Rosenberg, Stephen I. Katz, James J. Mulé

Research output: Contribution to journalArticle

Abstract

We examined the ability of epidermal Langerhans cells and splenic dendritic cells to present tumor-associated antigens (Ag) to immune T cells. Methylcholanthrene (MCA)-induced subcutaneous fibrosarcomas derived from C57BL/6 mice were used as tumor models. Our data demonstrate that both murine Langerhans cells and splenic dendritic cells have the capacity to present tumor-associated Ag to primed T cells. We found that variously treated tumor preparations (irradiated viable tumor cells, irradiated frozen-stored tumor cells, mitomycin C-treated viable tumor cells, and snap freeze-thawed tumor cell lysates) can be utilized for tumor Ag-pulsing. Primed CD4+ T cells demonstrated in vitro specificity towards their respective tumors and did not cross-react to other syngeneic MCA-induced or non-MCA-induced tumors. The T cell proliferative response critically depended on the presence of immune CD4+ T cells. We discuss the implications of these findings for the adoptive immunotherapy of cancer using immune CD4+ T cells.

Original languageEnglish (US)
Pages (from-to)315-319
Number of pages5
JournalEuropean Journal of Immunology
Volume24
Issue number2
StatePublished - Feb 1994
Externally publishedYes

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Langerhans Cells
Neoplasm Antigens
Dendritic Cells
T-Lymphocytes
Neoplasms
Methylcholanthrene
Adoptive Immunotherapy
Fibrosarcoma
Mitomycin
Inbred C57BL Mouse

Keywords

  • Dendritic cells
  • Langerhans cells
  • Tumor immunity
  • Tumor-associated antigens

ASJC Scopus subject areas

  • Immunology

Cite this

Cohen, P. J., Cohen, P. A., Rosenberg, S. A., Katz, S. I., & Mulé, J. J. (1994). Murine epidermal Langerhans cells and splenic dendritic cells present tumor-associated antigens to primed T cells. European Journal of Immunology, 24(2), 315-319.

Murine epidermal Langerhans cells and splenic dendritic cells present tumor-associated antigens to primed T cells. / Cohen, Philip J.; Cohen, Peter A.; Rosenberg, Steven A.; Katz, Stephen I.; Mulé, James J.

In: European Journal of Immunology, Vol. 24, No. 2, 02.1994, p. 315-319.

Research output: Contribution to journalArticle

Cohen, PJ, Cohen, PA, Rosenberg, SA, Katz, SI & Mulé, JJ 1994, 'Murine epidermal Langerhans cells and splenic dendritic cells present tumor-associated antigens to primed T cells', European Journal of Immunology, vol. 24, no. 2, pp. 315-319.
Cohen, Philip J. ; Cohen, Peter A. ; Rosenberg, Steven A. ; Katz, Stephen I. ; Mulé, James J. / Murine epidermal Langerhans cells and splenic dendritic cells present tumor-associated antigens to primed T cells. In: European Journal of Immunology. 1994 ; Vol. 24, No. 2. pp. 315-319.
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