Multistep Engineering of Pyrrolysyl-tRNA Synthetase to Genetically Encode Nε-(o-Azidobenzyloxycarbonyl) lysine for Site-Specific Protein Modification

Tatsuo Yanagisawa, Ryohei Ishii, Ryuya Fukunaga, Takatsugu Kobayashi, Kensaku Sakamoto, Shigeyuki Yokoyama

Research output: Contribution to journalArticle

Abstract

Pyrrolysyl-tRNA synthetase (PylRS) esterifies pyrrolysine to tRNAPyl. In this study, Nε-(tert-butyloxycarbonyl)-L-lysine (BocLys) and Nε-allyloxycarbonyl-L-lysine (AlocLys) were esterified to tRNAPyl by PylRS. Crystal structures of a PylRS catalytic fragment complexed with BocLys and an ATP analog and with AlocLys-AMP revealed that PylRS requires an Nε-carbonyl group bearing a substituent with a certain size. A PylRS(Y384F) mutant obtained by random screening exhibited higher in vitro aminoacylation and in vivo amber suppression activities with BocLys, AlocLys, and pyrrolysine than those of the wild-type PylRS. Furthermore, the structure-based Y306A mutation of PylRS drastically increased the in vitro aminoacylation activity for Nε-benzyloxycarbonyl-L-lysine (ZLys). A PylRS with both the Y306A and Y384F mutations enabled the large-scale preparation (>10 mg per liter medium) of proteins site-specifically containing Nε-(o-azidobenzyloxycarbonyl)-L-lysine (AzZLys). The AzZLys-containing protein was labeled with a fluorescent probe, by Staudinger ligation.

Original languageEnglish (US)
Pages (from-to)1187-1197
Number of pages11
JournalChemistry and Biology
Volume15
Issue number11
DOIs
StatePublished - Nov 24 2008
Externally publishedYes

Fingerprint

Amino Acyl-tRNA Synthetases
Lysine
Proteins
Aminoacylation
Bearings (structural)
Amber
Mutation
Adenosine Monophosphate
Fluorescent Dyes
Ligation
Screening
Adenosine Triphosphate
Crystal structure

Keywords

  • CHEMBIO
  • DNA
  • PROTEINS

ASJC Scopus subject areas

  • Biochemistry
  • Drug Discovery
  • Molecular Biology
  • Clinical Biochemistry
  • Molecular Medicine
  • Pharmacology

Cite this

Multistep Engineering of Pyrrolysyl-tRNA Synthetase to Genetically Encode Nε-(o-Azidobenzyloxycarbonyl) lysine for Site-Specific Protein Modification. / Yanagisawa, Tatsuo; Ishii, Ryohei; Fukunaga, Ryuya; Kobayashi, Takatsugu; Sakamoto, Kensaku; Yokoyama, Shigeyuki.

In: Chemistry and Biology, Vol. 15, No. 11, 24.11.2008, p. 1187-1197.

Research output: Contribution to journalArticle

Yanagisawa, Tatsuo ; Ishii, Ryohei ; Fukunaga, Ryuya ; Kobayashi, Takatsugu ; Sakamoto, Kensaku ; Yokoyama, Shigeyuki. / Multistep Engineering of Pyrrolysyl-tRNA Synthetase to Genetically Encode Nε-(o-Azidobenzyloxycarbonyl) lysine for Site-Specific Protein Modification. In: Chemistry and Biology. 2008 ; Vol. 15, No. 11. pp. 1187-1197.
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