Multiscale Optical Ca2+ Imaging of Tonal Organization in Mouse Auditory Cortex

John B. Issa, Benjamin D. Haeffele, Amit Agarwal, Dwight E. Bergles, Eric D. Young, David T. Yue

Research output: Contribution to journalArticlepeer-review

Abstract

Spatial patterns of functional organization, resolved by microelectrode mapping, comprise a core principle of sensory cortices. In auditory cortex, however,recent two-photon Ca2+ imaging challenges this precept, as the traditional tonotopic arrangementappears weakly organized at the level of individual neurons. To resolve this fundamental ambiguity about the organization of auditory cortex, we developed multiscale optical Ca2+ imaging of unanesthetized GCaMP transgenic mice. Single-neuron activity monitored by two-photon imaging was precisely registered to large-scale cortical maps provided bytranscranial widefield imaging. Neurons in theprimary field responded well to tones; neighboringneurons were appreciably cotuned, and preferred frequencies adhered tightly to a tonotopic axis. Bycontrast, nearby secondary-field neurons exhibited heterogeneous tuning. The multiscale imaging approach also readily localized vocalizationregions and neurons. Altogether, these findings cohere electrode and two-photon perspectives, resolve new features of auditory cortex, and offer apromising approach generalizable to any cortical area.

Original languageEnglish (US)
Pages (from-to)944-959
Number of pages16
JournalNeuron
Volume83
Issue number4
DOIs
StatePublished - Aug 20 2014

ASJC Scopus subject areas

  • Neuroscience(all)

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