Multipotency of human neural stem cells from fetal striatum

Yang Li, Xiao Chun Chi, Xiao Xia Li, Jin Chong Xu

Research output: Contribution to journalArticlepeer-review

Abstract

We had reported that neural stem cells from human fetal striatum (hsNSCs) expressed neural stem cell markers, and were capable of differentiation into neurons, astrocytes, and oligodendrocytes in vitro. To examine multipotency of hsNSCs, some experiments of transgerm layer differentiation in vitro were carried out. Our data indicated that hsNSCs could also generate osteocytes, adipocytes, and hepatocyte-like cells in vitro. Meanwhile, we injected hsNSCs into murine blastocysts at embryonic day 3.5 of gestation. Microinjection of hsNSCs led to the generation of chimeric embryos. Embryos at embryonic day 3.5 of gestation were shown to contribute to the hsNSC-derived cells by PCR-southern blot of 17αmod, a special method to discover human cells from animals. Analysis of the donor distribution in different tissues showed that donor-derived cells seeded to various tissues. The cellular nature of the human donor cells in chimeric tissues is, however, currently unknown, and further work will be done to identify what differentiated phenotypes have developed from the human cells.

Original languageEnglish (US)
Pages (from-to)1679-1683
Number of pages5
JournalNeuroreport
Volume19
Issue number17
DOIs
StatePublished - Nov 19 2008
Externally publishedYes

Keywords

  • Chimera
  • Differentiation
  • Human neural stem cells
  • Multipotency

ASJC Scopus subject areas

  • Neuroscience(all)

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