Multiplexed hybridization detection with multicolor colocalization of quantum dot nanoprobes

Yi Ping Ho, Matthew C. Kung, Samuel Yang, Tza Huei Wang

Research output: Contribution to journalArticle

Abstract

We demonstrate a hybridization detection method using multicolor oligonucleotide-functionalized quantum dots as nanoprobes. In the presence of various target sequences, combinatorial self-assembly of the nanoprobes via independent hybridization reactions leads to the generation of discernible sequence-specific spectral codings. Detection of single-molecule hybridization is achieved by measuring colocalization of individual nanoprobes. Genetic analysis for anthrax pathogenicity through simultaneous detection of multiple relevant sequences is demonstrated using this novel biosensing method as proof-of-concept.

Original languageEnglish (US)
Pages (from-to)1693-1697
Number of pages5
JournalNano Letters
Volume5
Issue number9
DOIs
StatePublished - Sep 2005

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Nanoprobes
Semiconductor quantum dots
quantum dots
oligonucleotides
Oligonucleotides
Self assembly
self assembly
coding
Molecules
molecules

ASJC Scopus subject areas

  • Materials Science(all)
  • Electronic, Optical and Magnetic Materials
  • Chemistry (miscellaneous)

Cite this

Multiplexed hybridization detection with multicolor colocalization of quantum dot nanoprobes. / Ho, Yi Ping; Kung, Matthew C.; Yang, Samuel; Wang, Tza Huei.

In: Nano Letters, Vol. 5, No. 9, 09.2005, p. 1693-1697.

Research output: Contribution to journalArticle

Ho, Yi Ping ; Kung, Matthew C. ; Yang, Samuel ; Wang, Tza Huei. / Multiplexed hybridization detection with multicolor colocalization of quantum dot nanoprobes. In: Nano Letters. 2005 ; Vol. 5, No. 9. pp. 1693-1697.
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