Multiplexed analysis of retinal gene expression and chromatin accessibility using scrna-seq and scatac-seq

Kurt Weir, Patrick Leavey, Clayton Santiago, Seth Blackshaw

Research output: Contribution to journalArticlepeer-review

Abstract

Powerful next generation sequencing techniques offer robust and comprehensive analysis to investigate how retinal gene regulatory networks function during development and in disease states. Single-cell RNA sequencing allows us to comprehensively profile gene expression changes observed in retinal development and disease at a cellular level, while single-cell ATAC-Seq allows analysis of chromatin accessibility and transcription factor binding to be profiled at similar resolution. Here the use of these techniques in the developing retina is described, and MULTI-Seq is demonstrated, where individual samples are labeled with a modified oligonucleotide-lipid complex, enabling researchers to both increase the scope of individual experiments and substantially reduce costs.

Original languageEnglish (US)
Article numbere62239
JournalJournal of Visualized Experiments
Volume2021
Issue number169
DOIs
StatePublished - 2021

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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