Multiplex STR and mitochondrial DNA testing for paraffin embedded specimen of healthy and malignant tissue: Interpretation issues

C. Lu, Z. Budimlija, D. Popiolek, P. Illei, B. West, F. Fogt, J. J. Wei, M. Prinz

Research output: Contribution to journalArticle

Abstract

STR typing results for 70 pairs of healthy vs. malignant tissues demonstrate the occurrence of microsatellite instability (MSI) and loss of heterozygosity (LOH) in the tumor samples. In order to not misinterpret degradation effects as LOH, this study recommends to only interpret alleles ≤ 350 bp with a peak intensity > 300 RFU. mtDNA data for the same samples did not reveal increased mutation rates but for many cases were affected by DNA contamination. The mtDNA assay can be valuable in confirming an inclusion. Overall these sample types can still be used for identification purposes since any observed discrepancies can be explained.

Original languageEnglish (US)
Pages (from-to)648-650
Number of pages3
JournalInternational Congress Series
Volume1288
DOIs
StatePublished - Apr 1 2006
Externally publishedYes

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Keywords

  • Allelic instability
  • Human identification
  • Loss of heterozygosity
  • Malignant tumors
  • STR
  • mtDNA

ASJC Scopus subject areas

  • Medicine(all)

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