Multiplex pcr to detect pampc β-lactamases among enterobacteriaceae at a tertiary care laboratory in Mumbai, India

Mubin Kazi, Kanchan Ajbani, Jeffrey A. Tornheim, Anjali Shetty, Camilla Rodrigues

Research output: Contribution to journalArticlepeer-review

Abstract

Drug-resistance due to AmpC β-lactamases represents a growing problem worldwide. In this study, a previously collected sample of 108 cefoxitin-resistant clinical isolates was assessed for AmpC b-lactamase production through routine phenotypic testing and double-disc cefoxitin/cloxcallin (DD-CC), cefoxitin/phenylboronic acid (CDT-PBA) and AmpC disc tests. The same isolates were characterized by a novel multiplex polymerase chain reaction molecular assay to detect the presence of bla ACT , bla DHA , bla CIT , bla FOX , bla MIR and bla MOX . By phenotypic analysis, 56%, 55% and 48 % were detected as being AmpC β-lactamase producers by the CDT-PBA, DD-CC and AmpC disc tests, respectively. By molecular analysis, 57 % were determined to be AmpC β-lactamase producers, including 34 % bla FOX , 8 % bla CIT and 1.6 % bla DHA as mono-AmpC producers. The production of multiple AmpC molecular types was common, including 30 % with both bla CIT+FOX and 1.6 % each of bla CIT+DHA , bla ACT+MIR , bla ACT+FOX , bla ACT+DHA and bla MIR+FOX . Molecular characterization of AmpC would help detect the prevalence of AmpC β-lactamase producers, facilitate proper patient management and implement infection control practices.

Original languageEnglish (US)
Article number000748
Pages (from-to)246-250
Number of pages5
JournalMicrobiology (United Kingdom)
Volume165
Issue number2
DOIs
StatePublished - Feb 2019

Keywords

  • AmpC disk test
  • AmpC β-lactamases
  • Cefoxitin-resistance
  • Cefoxtin-Cloxcallin
  • Molecular characterization

ASJC Scopus subject areas

  • Microbiology

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