Multiple types of cerebellar target neurons and their circuitry in the vestibule-ocular reflex

Minyoung Shin, Setareh H. Moghadam, Chris Sekirnjak, Martha W. Bagnall, Kristine E. Kolkman, Richard Jacobs, Michael Faulstich, Sascha du Lac

Research output: Contribution to journalArticle

Abstract

The cerebellum influences behavior and cognition exclusively via Purkinje cell synapses onto neurons in the deep cerebellar and vestibular nuclei. In contrast with the rich information available about the organization of the cerebellar cortex and its synaptic inputs, relatively little is known about microcircuitry postsynaptic to Purkinje cells. Here we examined the cell types and microcircuits through which Purkinje cells influence an oculomotor behavior controlled by the cerebellum, the horizontal vestibule-ocular reflex, which involves only two eye muscles. Usingacombinationofanatomical tracing and electrophysiological recordingsintransgenic mouse lines, we identified several classes of neurons in the medial vestibular nucleus that receive Purkinje cell synapses from the cerebellar flocculus. Glycinergic and glutamatergic flocculus target neurons (FTNs) with somata densely surrounded by Purkinje cell terminals projected axonsto the ipsilateral abducens and oculomotor nuclei, respectively.Of three additional types ofFTNs that were sparsely innervated by Purkinje cells, glutamatergic and glycinergic neurons projected to the contralateral and ipsilateral abducens, respectively, and GABAe-rgic neurons projected to contralateral vestibular nuclei. Densely innervated FTNs had high spontaneous firing rates and pronounced postinhibitory rebound firing, and were physiologically homogeneous, whereas the intrinsic excitability of sparsely innervated FTNs varied widely. Heterogeneity in the molecular expression, physiological properties, and postsynaptic targets of FTNs implies that Pur-kinje cell activity influences the neural control of eye movements in several distinct ways. These results indicate that the cerebellum regulates a simple reflex behavior via at least five different cell types that are postsynaptic to Purkinje cells.

Original languageEnglish (US)
Pages (from-to)10776-10786
Number of pages11
JournalJournal of Neuroscience
Volume31
Issue number30
DOIs
StatePublished - Jul 27 2011
Externally publishedYes

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Purkinje Cells
Reflex
Neurons
Vestibular Nuclei
Cerebellum
Synapses
Cerebellar Nuclei
Cerebellar Cortex
Carisoprodol
Eye Movements
Cognition
Muscles

ASJC Scopus subject areas

  • Neuroscience(all)

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Multiple types of cerebellar target neurons and their circuitry in the vestibule-ocular reflex. / Shin, Minyoung; Moghadam, Setareh H.; Sekirnjak, Chris; Bagnall, Martha W.; Kolkman, Kristine E.; Jacobs, Richard; Faulstich, Michael; du Lac, Sascha.

In: Journal of Neuroscience, Vol. 31, No. 30, 27.07.2011, p. 10776-10786.

Research output: Contribution to journalArticle

Shin, M, Moghadam, SH, Sekirnjak, C, Bagnall, MW, Kolkman, KE, Jacobs, R, Faulstich, M & du Lac, S 2011, 'Multiple types of cerebellar target neurons and their circuitry in the vestibule-ocular reflex', Journal of Neuroscience, vol. 31, no. 30, pp. 10776-10786. https://doi.org/10.1523/JNEUROSCI.0768-11.2011
Shin M, Moghadam SH, Sekirnjak C, Bagnall MW, Kolkman KE, Jacobs R et al. Multiple types of cerebellar target neurons and their circuitry in the vestibule-ocular reflex. Journal of Neuroscience. 2011 Jul 27;31(30):10776-10786. https://doi.org/10.1523/JNEUROSCI.0768-11.2011
Shin, Minyoung ; Moghadam, Setareh H. ; Sekirnjak, Chris ; Bagnall, Martha W. ; Kolkman, Kristine E. ; Jacobs, Richard ; Faulstich, Michael ; du Lac, Sascha. / Multiple types of cerebellar target neurons and their circuitry in the vestibule-ocular reflex. In: Journal of Neuroscience. 2011 ; Vol. 31, No. 30. pp. 10776-10786.
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