Multiple-pulse-mediated electrofusion of intact erythrocyte onto human term placental amnion

Subrata Biswas, Sujoy K. Guha

Research output: Contribution to journalArticle

Abstract

The creation of surface modified human term placental amnion by electrofusing human cells onto its surface has been thought of. A multiple-pulse electrofusion protocol with 10 square pulses of 10-μs pulse length, and electric field of 0.2 kV cm-1, can make erythrocyte-amnion tissue electrofusion possible. The protocol devised merge the cell-tissue-adherence steps with fusogenic pulse. The finding opens up a new avenue of cell electrofusion onto human tissue with minimal procedural complexities. Copyright (C) 1999 Elsevier Science S.A.

Original languageEnglish (US)
Pages (from-to)431-434
Number of pages4
JournalBioelectrochemistry and Bioenergetics
Volume48
Issue number2
DOIs
StatePublished - May 1999
Externally publishedYes

Fingerprint

Amnion
erythrocytes
Erythrocytes
Tissue
pulses
cells
Cells
Electric fields
electric fields

Keywords

  • Cell-tissue electrofusion
  • Graft tissue
  • Microvilli
  • Placental amnion

ASJC Scopus subject areas

  • Biochemistry

Cite this

Multiple-pulse-mediated electrofusion of intact erythrocyte onto human term placental amnion. / Biswas, Subrata; Guha, Sujoy K.

In: Bioelectrochemistry and Bioenergetics, Vol. 48, No. 2, 05.1999, p. 431-434.

Research output: Contribution to journalArticle

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