Multiple-pulse-mediated electrofusion of intact erythrocyte onto human term placental amnion

Subrata Biswas, Sujoy K. Guha

Research output: Contribution to journalArticle

Abstract

The creation of surface modified human term placental amnion by electrofusing human cells onto its surface has been thought of. A multiple-pulse electrofusion protocol with 10 square pulses of 10-μs pulse length, and electric field of 0.2 kV cm-1, can make erythrocyte-amnion tissue electrofusion possible. The protocol devised merge the cell-tissue-adherence steps with fusogenic pulse. The finding opens up a new avenue of cell electrofusion onto human tissue with minimal procedural complexities. Copyright (C) 1999 Elsevier Science S.A.

Original languageEnglish (US)
Pages (from-to)431-434
Number of pages4
JournalBioelectrochemistry and Bioenergetics
Volume48
Issue number2
DOIs
StatePublished - May 1 1999

Keywords

  • Cell-tissue electrofusion
  • Graft tissue
  • Microvilli
  • Placental amnion

ASJC Scopus subject areas

  • Biophysics
  • Physical and Theoretical Chemistry
  • Electrochemistry

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