Multiple portions of poly(A)-binding protein stimulate translation in vivo

Nicola K. Gray, Jeffery M. Coller, Kirsten S. Dickson, Marvin Wickens

Research output: Contribution to journalArticlepeer-review

182 Scopus citations

Abstract

Translational stimulation of mRNAs during early development is often accompanied by increases in poly(A) tail length. Poly(A)-binding protein (PAB) is an evolutionarily conserved protein that binds to the poly(A) tails of eukaryotic mRNAs. We examined PAB's role in living cells, using both Xenopus laevis oocytes and Saccharomyces cerevisiae, by tethering it to the 3'-untranslated region of reporter mRNAs. Tethered PAB stimulates translation in vivo. Neither a poly(A) tail nor PAB's poly(A)-binding activity is required. Multiple domains of PAB act redundantly in oocytes to stimulate translation: the interaction of RNA recognition motifs (RRMs) 1 and 2 with eukaryotic initiation factor-4G correlates with translational stimulation. Interaction with Paip-1 is insufficient for stimulation. RRMs 3 and 4 also stimulate, but bind neither factor. The regions of tethered PAB required in yeast to stimulate translation and stabilize mRNAs differ, implying that the two functions are distinct. Our results establish that oocytes contain the machinery necessary to support PAB-mediated translation and suggest that PAB may be an important participant in translational regulation during early development.

Original languageEnglish (US)
Pages (from-to)4723-4733
Number of pages11
JournalEMBO Journal
Volume19
Issue number17
DOIs
StatePublished - Sep 1 2000
Externally publishedYes

Keywords

  • Early development
  • Poly(A)
  • Translation
  • Xenopus oocytes
  • mRNA turnover

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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