Multiple nuclear factors interact with the immunoglobulin enhancer sequences

Ranjan Sen, David Baltimore

Research output: Contribution to journalArticlepeer-review

Abstract

To characterize proteins that bind to the immunoglobulin (Ig) heavy chain and the κ light chain enhancers, an electrophoretic mobility shift assay with end-labeled DNA fragments was used. Three binding proteins have been found. One is NF-A, a factor found in all tested cell types that binds to the octamer sequence found upstream of all Ig variable region gene segments and to the same octamer in the heavy chain enhancer. The second, also ubiquitous, protein binds to a sequence in both the heavy chain and the κ enhancers that was previously shown to be protected from methylation in vivo. Other closely related sites do not compete for this binding, implying a restriction enzyme-like binding specificity. The third protein binds to a sequence in the κ enhancer (and to an identical sequence in the SV40 enhancer) and is restricted in its occurrence to B cells.

Original languageEnglish (US)
Pages (from-to)705-716
Number of pages12
JournalCell
Volume46
Issue number5
DOIs
StatePublished - Aug 29 1986
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Medicine(all)

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