Multiple isoforms of metallothionein are expressed in the porcine liver

Min Chuan Huang, Peter K. Pan, Zu Fan Zheng, Nick C. Chen, Joyce Y. Peng, P C Huang

Research output: Contribution to journalArticle

Abstract

Isogenes are highly homologous to each other and are often difficult to ascertain, as has been the case with metallothionein, a metal-binding protein rich in cysteines. Conventional separation of metallothionein isoforms relied on ion exchange chromatography of the proteins, or screening for the sequences from gene libraries. In this study, a combination of RT-PCR and partial protein sequencing is used in the identification of metallothionein isogenes expressed in porcine liver. By this approach, we have identified expressed coding sequences which constitute 10 new isogenes. Of the four known groups of metallothioneins (MT), phylogenetic analyses place these pig isogenes in the MT-1 group, except two which are identified as being closely related to MT-2, and none in groups 3 and 4. The isogenes are thus named pMT-1a to -1g, and pMT-2a and -2b. While each of the isogene sequences is unique, two isogenes, pMT-le1 and pMT-le2, share an identical amino acid sequence, differing only in specific codons. Two others, pMT-1b and pMT-1g, have a cysteine substituted by arginine, the first such sequence ever detected in MT. pMT-2a and pMT-2b are closely aligned with the MT-2 group of vertebrates, in spite of the absence of a characteristic acidic amino acid at position 10 or 11, common in other mammalian metallothioneins.

Original languageEnglish (US)
Pages (from-to)49-55
Number of pages7
JournalGene
Volume211
Issue number1
DOIs
StatePublished - Apr 28 1998

Fingerprint

Metallothionein
Protein Isoforms
Swine
Liver
Cysteine
Acidic Amino Acids
Protein Sequence Analysis
Ion Exchange Chromatography
Gene Library
Codon
Arginine
Vertebrates
Amino Acid Sequence
Carrier Proteins
Metals
Polymerase Chain Reaction

Keywords

  • cDNA
  • Gene family
  • HPLC
  • RT-PCR

ASJC Scopus subject areas

  • Genetics

Cite this

Huang, M. C., Pan, P. K., Zheng, Z. F., Chen, N. C., Peng, J. Y., & Huang, P. C. (1998). Multiple isoforms of metallothionein are expressed in the porcine liver. Gene, 211(1), 49-55. https://doi.org/10.1016/S0378-1119(98)00067-5

Multiple isoforms of metallothionein are expressed in the porcine liver. / Huang, Min Chuan; Pan, Peter K.; Zheng, Zu Fan; Chen, Nick C.; Peng, Joyce Y.; Huang, P C.

In: Gene, Vol. 211, No. 1, 28.04.1998, p. 49-55.

Research output: Contribution to journalArticle

Huang, MC, Pan, PK, Zheng, ZF, Chen, NC, Peng, JY & Huang, PC 1998, 'Multiple isoforms of metallothionein are expressed in the porcine liver', Gene, vol. 211, no. 1, pp. 49-55. https://doi.org/10.1016/S0378-1119(98)00067-5
Huang, Min Chuan ; Pan, Peter K. ; Zheng, Zu Fan ; Chen, Nick C. ; Peng, Joyce Y. ; Huang, P C. / Multiple isoforms of metallothionein are expressed in the porcine liver. In: Gene. 1998 ; Vol. 211, No. 1. pp. 49-55.
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