Multiple gating modes and the effect of modulating factors on the μI sodium channel

Jiuying Zhou; Jerald F. Potts; James S. Trimmer; William S. Agnew; Fred J. Sigworth

doi:10.1016/0896-6273(91)90280-D

Multiple gating modes and the effect of modulating factors on the μI sodium channel

Jiuying Zhou, Jerald F. Potts, James S. Trimmer, William S. Agnew, Fred J. Sigworth

Research output: Contribution to journal › Article › peer-review

121 Scopus citations

Abstract

Macroscopic current from the μI skeletal muscle sodium channel expressed in Xenopus oocytes shows inactivation with two exponential components. The major, slower component's amplitude decreases with rapid pulsing. When μI cRNA is coinjected with rat skeletal muscle or brain mRNA the faster component becomes predominant. Individual μI channels switch between two principal gating modes, opening either only once per depolarization, or repeatedly in long bursts. These two modes differ in both activation and inactivation kinetics. There is also evidence for additional gating modes. It appears that the equilibrium among gating modes is influenced by a modulating factor encoded in rat rat skeletal muscle and brain mRNA. The modal gating is similar to that observed in hyperkalemic periodic paralysis.

Original language	English (US)
Pages (from-to)	775-785
Number of pages	11
Journal	Neuron
Volume	7
Issue number	5
DOIs	https://doi.org/10.1016/0896-6273(91)90280-D
State	Published - Nov 1991
Externally published	Yes

ASJC Scopus subject areas

Neuroscience(all)

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10.1016/0896-6273(91)90280-D

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@article{130e10b8f7bb45d4a9a780458989c541,

title = "Multiple gating modes and the effect of modulating factors on the μI sodium channel",

abstract = "Macroscopic current from the μI skeletal muscle sodium channel expressed in Xenopus oocytes shows inactivation with two exponential components. The major, slower component's amplitude decreases with rapid pulsing. When μI cRNA is coinjected with rat skeletal muscle or brain mRNA the faster component becomes predominant. Individual μI channels switch between two principal gating modes, opening either only once per depolarization, or repeatedly in long bursts. These two modes differ in both activation and inactivation kinetics. There is also evidence for additional gating modes. It appears that the equilibrium among gating modes is influenced by a modulating factor encoded in rat rat skeletal muscle and brain mRNA. The modal gating is similar to that observed in hyperkalemic periodic paralysis.",

author = "Jiuying Zhou and Potts, {Jerald F.} and Trimmer, {James S.} and Agnew, {William S.} and Sigworth, {Fred J.}",

note = "Funding Information: We thank Drs. M. Hollman and S. Heinemann for providing F12 cRNA used in this study. Wealso thank Sheila Crean for excellent technical assistanceand Chinweike Ukomadu, Nathan Schoppa, and Scott Shenkel for helpful discussions. This work is supported by National Institutes of Health grant HL38156 to Funding Information: W. S. A. and F. J. S. and grants from the Muscular Dystrophy Association and National Multiple Sclerosis Society to W. S. A.",

year = "1991",

month = nov,

doi = "10.1016/0896-6273(91)90280-D",

language = "English (US)",

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pages = "775--785",

journal = "Neuron",

issn = "0896-6273",

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T1 - Multiple gating modes and the effect of modulating factors on the μI sodium channel

AU - Zhou, Jiuying

AU - Potts, Jerald F.

AU - Trimmer, James S.

AU - Agnew, William S.

AU - Sigworth, Fred J.

N1 - Funding Information: We thank Drs. M. Hollman and S. Heinemann for providing F12 cRNA used in this study. Wealso thank Sheila Crean for excellent technical assistanceand Chinweike Ukomadu, Nathan Schoppa, and Scott Shenkel for helpful discussions. This work is supported by National Institutes of Health grant HL38156 to Funding Information: W. S. A. and F. J. S. and grants from the Muscular Dystrophy Association and National Multiple Sclerosis Society to W. S. A.

PY - 1991/11

Y1 - 1991/11

N2 - Macroscopic current from the μI skeletal muscle sodium channel expressed in Xenopus oocytes shows inactivation with two exponential components. The major, slower component's amplitude decreases with rapid pulsing. When μI cRNA is coinjected with rat skeletal muscle or brain mRNA the faster component becomes predominant. Individual μI channels switch between two principal gating modes, opening either only once per depolarization, or repeatedly in long bursts. These two modes differ in both activation and inactivation kinetics. There is also evidence for additional gating modes. It appears that the equilibrium among gating modes is influenced by a modulating factor encoded in rat rat skeletal muscle and brain mRNA. The modal gating is similar to that observed in hyperkalemic periodic paralysis.

AB - Macroscopic current from the μI skeletal muscle sodium channel expressed in Xenopus oocytes shows inactivation with two exponential components. The major, slower component's amplitude decreases with rapid pulsing. When μI cRNA is coinjected with rat skeletal muscle or brain mRNA the faster component becomes predominant. Individual μI channels switch between two principal gating modes, opening either only once per depolarization, or repeatedly in long bursts. These two modes differ in both activation and inactivation kinetics. There is also evidence for additional gating modes. It appears that the equilibrium among gating modes is influenced by a modulating factor encoded in rat rat skeletal muscle and brain mRNA. The modal gating is similar to that observed in hyperkalemic periodic paralysis.

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JO - Neuron

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Multiple gating modes and the effect of modulating factors on the μI sodium channel

Abstract

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