Multiple EGFR ligands participate in guiding migrating border cells

Jocelyn A. McDonald, Elaine M. Pinheiro, Lisa Kadlec, Trudi Schupbach, Denise J. Montell

Research output: Contribution to journalArticle

Abstract

Cell migration is an important feature of embryonic development as well as tumor metastasis. Border cells in the Drosophila ovary have emerged as a useful in vivo model for uncovering the molecular mechanisms that control many aspects of cell migration including guidance. It was previously shown that two receptor tyrosine kinases, epidermal growth factor receptor (EGFR) and PDGF- and VEGF-related receptor (PVR), together contribute to border cell migration. Whereas the ligand for PVR, PVF1, is known to guide border cells, it is unclear which of the four activating EGFR ligands function in this process. We developed an assay to detect the ability of secreted factors to reroute migrating border cells in vivo and tested the activity of EGFR ligands compared to PVF1. Two ligands, Keren and Spitz, guided border cells whereas the other ligands, Gurken and Vein, did not. In addition, only Keren and Spitz were expressed at the appropriate stage in the oocyte, the target of border cell migration. Therefore, a complex combination of EGFR and PVR ligands together guide border cells to the oocyte.

Original languageEnglish (US)
Pages (from-to)94-103
Number of pages10
JournalDevelopmental biology
Volume296
Issue number1
DOIs
StatePublished - Aug 1 2006

Keywords

  • Border cells
  • Drosophila melanogaster
  • EGFR
  • Gurken
  • Keren
  • Migration
  • PVF1
  • PVR
  • Spitz
  • Vein

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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  • Cite this

    McDonald, J. A., Pinheiro, E. M., Kadlec, L., Schupbach, T., & Montell, D. J. (2006). Multiple EGFR ligands participate in guiding migrating border cells. Developmental biology, 296(1), 94-103. https://doi.org/10.1016/j.ydbio.2006.04.438