Multiple assay characterization of murine monoclonal antimelanoma antibodies

G. M. Stuhlmiller, Michael J Borowitz, B. P. Croker, H. F. Seigler

Research output: Contribution to journalArticle

Abstract

Selected murine monoclonal antimelanoma antibodies have been extensively evaluated using multiple radioimmunoassay methods, immunoprecipitation and immunohistochemistry. Use of this approach has permitted more complete definition of the specificity of these reagents and provided information regarding the nature and distribution of the respective tumor associated antigens (TAA). Several patterns of reactivity were identified. Some of the reagents were highly reactive with melanomas but also with a variety of tissues of nonmelanoma origin. Others were less highly reactive but of greater specificity for melanoma. Finally, certain of the reagents were poorly reactive in the assays utilized or demonstrated assay-dependent reactivity. None of the included monoclonal antibodies appeared to detect TAA restricted in distribution solely to melanoma.

Original languageEnglish (US)
Pages (from-to)447-460
Number of pages14
JournalHybridoma
Volume1
Issue number4
StatePublished - 1982
Externally publishedYes

Fingerprint

Melanoma
Monoclonal Antibodies
Neoplasm Antigens
Immunoprecipitation
Radioimmunoassay
Immunohistochemistry

ASJC Scopus subject areas

  • Genetics
  • Immunology

Cite this

Stuhlmiller, G. M., Borowitz, M. J., Croker, B. P., & Seigler, H. F. (1982). Multiple assay characterization of murine monoclonal antimelanoma antibodies. Hybridoma, 1(4), 447-460.

Multiple assay characterization of murine monoclonal antimelanoma antibodies. / Stuhlmiller, G. M.; Borowitz, Michael J; Croker, B. P.; Seigler, H. F.

In: Hybridoma, Vol. 1, No. 4, 1982, p. 447-460.

Research output: Contribution to journalArticle

Stuhlmiller, GM, Borowitz, MJ, Croker, BP & Seigler, HF 1982, 'Multiple assay characterization of murine monoclonal antimelanoma antibodies', Hybridoma, vol. 1, no. 4, pp. 447-460.
Stuhlmiller, G. M. ; Borowitz, Michael J ; Croker, B. P. ; Seigler, H. F. / Multiple assay characterization of murine monoclonal antimelanoma antibodies. In: Hybridoma. 1982 ; Vol. 1, No. 4. pp. 447-460.
@article{e93013765d4d4aff980705d025100d3d,
title = "Multiple assay characterization of murine monoclonal antimelanoma antibodies",
abstract = "Selected murine monoclonal antimelanoma antibodies have been extensively evaluated using multiple radioimmunoassay methods, immunoprecipitation and immunohistochemistry. Use of this approach has permitted more complete definition of the specificity of these reagents and provided information regarding the nature and distribution of the respective tumor associated antigens (TAA). Several patterns of reactivity were identified. Some of the reagents were highly reactive with melanomas but also with a variety of tissues of nonmelanoma origin. Others were less highly reactive but of greater specificity for melanoma. Finally, certain of the reagents were poorly reactive in the assays utilized or demonstrated assay-dependent reactivity. None of the included monoclonal antibodies appeared to detect TAA restricted in distribution solely to melanoma.",
author = "Stuhlmiller, {G. M.} and Borowitz, {Michael J} and Croker, {B. P.} and Seigler, {H. F.}",
year = "1982",
language = "English (US)",
volume = "1",
pages = "447--460",
journal = "Monoclonal Antibodies in Immunodiagnosis and Immunotherapy",
issn = "2167-9436",
publisher = "Mary Ann Liebert Inc.",
number = "4",

}

TY - JOUR

T1 - Multiple assay characterization of murine monoclonal antimelanoma antibodies

AU - Stuhlmiller, G. M.

AU - Borowitz, Michael J

AU - Croker, B. P.

AU - Seigler, H. F.

PY - 1982

Y1 - 1982

N2 - Selected murine monoclonal antimelanoma antibodies have been extensively evaluated using multiple radioimmunoassay methods, immunoprecipitation and immunohistochemistry. Use of this approach has permitted more complete definition of the specificity of these reagents and provided information regarding the nature and distribution of the respective tumor associated antigens (TAA). Several patterns of reactivity were identified. Some of the reagents were highly reactive with melanomas but also with a variety of tissues of nonmelanoma origin. Others were less highly reactive but of greater specificity for melanoma. Finally, certain of the reagents were poorly reactive in the assays utilized or demonstrated assay-dependent reactivity. None of the included monoclonal antibodies appeared to detect TAA restricted in distribution solely to melanoma.

AB - Selected murine monoclonal antimelanoma antibodies have been extensively evaluated using multiple radioimmunoassay methods, immunoprecipitation and immunohistochemistry. Use of this approach has permitted more complete definition of the specificity of these reagents and provided information regarding the nature and distribution of the respective tumor associated antigens (TAA). Several patterns of reactivity were identified. Some of the reagents were highly reactive with melanomas but also with a variety of tissues of nonmelanoma origin. Others were less highly reactive but of greater specificity for melanoma. Finally, certain of the reagents were poorly reactive in the assays utilized or demonstrated assay-dependent reactivity. None of the included monoclonal antibodies appeared to detect TAA restricted in distribution solely to melanoma.

UR - http://www.scopus.com/inward/record.url?scp=0020404297&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020404297&partnerID=8YFLogxK

M3 - Article

VL - 1

SP - 447

EP - 460

JO - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy

JF - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy

SN - 2167-9436

IS - 4

ER -