Multiple aspects of male germ cell development and interactions with Sertoli cells require inositol hexakisphosphate kinase-1

Chenglai Fu, Tomas Rojas, Alfred C. Chin, Weiwei Cheng, Isaac A. Bernstein, Lauren K. Albacarys, William W Wright, Solomon H Snyder

Research output: Contribution to journalArticle

Abstract

Inositol hexakisphosphate kinase-1 (IP6K1) is required for male fertility, but the underlying mechanisms have been elusive. Here, we report that IP6K1 is required for multiple aspects of male germ cell development. This development requires selective interactions between germ cells and Sertoli cells, namely apical ectoplasmic specialization. Spermiation (sperm release) requires tubulobulbar complexes. We found that the apical ectoplasmic specialization and tubulobulbar complexes were poorly formed or disrupted in IP6K1 KOs. Deletion of IP6K1 elicited several aberrations, including: 1, sloughing off of round germ cells; 2, disorientation and malformation of elongating/elongated spermatids; 3, degeneration of acrosomes; 4, defects in germ-Sertoli cell interactions and 5, failure of spermiation. Eventually the sperm cells were not released but phagocytosed by Sertoli cells leading to an absence of sperm in the epididymis.

Original languageEnglish (US)
Article number7039
JournalScientific Reports
Volume8
Issue number1
DOIs
StatePublished - Dec 1 2018

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Sertoli Cells
Germ Cells
Cell Communication
Spermatozoa
Acrosome
Spermatids
Epididymis
Phagocytosis
Fertility
inositol hexakisphosphate kinase

ASJC Scopus subject areas

  • General

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Multiple aspects of male germ cell development and interactions with Sertoli cells require inositol hexakisphosphate kinase-1. / Fu, Chenglai; Rojas, Tomas; Chin, Alfred C.; Cheng, Weiwei; Bernstein, Isaac A.; Albacarys, Lauren K.; Wright, William W; Snyder, Solomon H.

In: Scientific Reports, Vol. 8, No. 1, 7039, 01.12.2018.

Research output: Contribution to journalArticle

Fu, Chenglai ; Rojas, Tomas ; Chin, Alfred C. ; Cheng, Weiwei ; Bernstein, Isaac A. ; Albacarys, Lauren K. ; Wright, William W ; Snyder, Solomon H. / Multiple aspects of male germ cell development and interactions with Sertoli cells require inositol hexakisphosphate kinase-1. In: Scientific Reports. 2018 ; Vol. 8, No. 1.
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AU - Wright, William W

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