Multimodal thin layer chromatographic separation of bryostatins 1 and 2 from Bugula neritina extracts employing side-by-side silica gel/C18 plates

H. J. Issaq, K. E. Seburn, P. Andrews, D. E. Schaufelberger

Research output: Contribution to journalArticlepeer-review

Abstract

The separation of bryostatin 1 from bryostatin 2 and other compounds from Bugula neritina extracts using silica gel/C18 side-by-side thin layer chromatography plates was accomplished. Clean-up and preliminary separation was carried out on the silica gel strip with a mobile phase of hexane:acetone 3:2, while resolution was achieved on the C18 side of the plate using 85% acetonitrile/water. Visualization of bryostatins 1 and 2 was achieved by spraying with anisaldehyde reagent and heating in an oven at 110°C.

Original languageEnglish (US)
Pages (from-to)3129-3134
Number of pages6
JournalJournal of Liquid Chromatography
Volume12
Issue number16
StatePublished - Dec 1989
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Medicine

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