Multilocus sequence typing of Pathogens

Marcos Pérez-Losada, Megan L. Porter, Raphael P. Viscidi, Keith A. Crandall

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Several molecular typing methods have been proposed that can identify isolates in localized disease outbreaks. The main standard for molecular typing is multilocus sequence typing (MLST), which is established on the population genetic concepts and methods of the multilocus enzyme electrophoresis. Now, MLST has become the standard approach for characterizing bacteria and some eukaryotes because it is more variable, portable, and reproducible than other typing methods. This chapter gives us detailed information about multilocus sequence typing pathogens. It emphasizes that the selected loci should be within housekeeping genes encoding proteins for core metabolic functions. The development of a website containing MLST databases to which public health and research communities could have access was one of the aims of MLST. The advantages and disadvantages of MLST are detailed in this chapter. The two basic strategies to the analysis of MLST data, that is, the Allele-Based Methods and Nucleotide-Based Methods are explained. MLST has gained widespread popularity as a typing method. This chapter concludes that epidemiological data and full-genome sequencing data can be used to identify phylogenetically informative loci across species.

Original languageEnglish (US)
Title of host publicationGenetics and Evolution of Infectious Diseases
PublisherElsevier Inc.
Pages503-521
Number of pages19
ISBN (Print)9780123848901
DOIs
StatePublished - Dec 1 2011

ASJC Scopus subject areas

  • Immunology and Microbiology(all)

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