Multilocus sequence typing (MLST) analysis of Vibrio cholerae O1 El Tor isolates from Mozambique that harbour the classical CTX prophage

Je Hee Lee, Kyung Ho Han, Seon Young Choi, Marcelino E S Lucas, C. Mondlane, M. Ansaruzzaman, G. Balakrish Nair, David A. Sack, Lorenz Von Seidlein, John D. Clemens, Manki Song, Jongsik Chun, Dong Wook Kim

Research output: Contribution to journalArticle

Abstract

Vibrio cholerae O1 isolates belonging to the Ogawa serotype, El Tor biotype, harbouring the classical CTX prophage were first isolated in Mozambique in 2004. Multilocus sequence typing (MLST) analysis using nine genetic loci showed that the Mozambique isolates have the same sequence type (ST) as O1 El Tor N16961, a representative of the current seventh cholera pandemic. Analysis of the CTX prophage in the Mozambique isolates indicated that there is one type of rstR in these isolates: the classical CTX prophage. It was also found that the ctxB-rstR-rstA-rstB-phs-cep fragment was PCR-amplified from these isolates, which indicates the presence of a tandem repeat of the classical CTX prophage in the genome of the Mozambique isolates. The possible origin of these isolates and the presence of the tandem repeat of the classical prophage in them implicate the presence of the classical CTX phage.

Original languageEnglish (US)
Pages (from-to)165-170
Number of pages6
JournalJournal of Medical Microbiology
Volume55
Issue number2
DOIs
StatePublished - Feb 2006
Externally publishedYes

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Vibrio cholerae O1
Mozambique
Prophages
Multilocus Sequence Typing
Tandem Repeat Sequences
Genetic Loci
Cholera
Pandemics
Bacteriophages
Genome
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Multilocus sequence typing (MLST) analysis of Vibrio cholerae O1 El Tor isolates from Mozambique that harbour the classical CTX prophage. / Lee, Je Hee; Han, Kyung Ho; Choi, Seon Young; Lucas, Marcelino E S; Mondlane, C.; Ansaruzzaman, M.; Nair, G. Balakrish; Sack, David A.; Von Seidlein, Lorenz; Clemens, John D.; Song, Manki; Chun, Jongsik; Kim, Dong Wook.

In: Journal of Medical Microbiology, Vol. 55, No. 2, 02.2006, p. 165-170.

Research output: Contribution to journalArticle

Lee, JH, Han, KH, Choi, SY, Lucas, MES, Mondlane, C, Ansaruzzaman, M, Nair, GB, Sack, DA, Von Seidlein, L, Clemens, JD, Song, M, Chun, J & Kim, DW 2006, 'Multilocus sequence typing (MLST) analysis of Vibrio cholerae O1 El Tor isolates from Mozambique that harbour the classical CTX prophage', Journal of Medical Microbiology, vol. 55, no. 2, pp. 165-170. https://doi.org/10.1099/jmm.0.46287-0
Lee, Je Hee ; Han, Kyung Ho ; Choi, Seon Young ; Lucas, Marcelino E S ; Mondlane, C. ; Ansaruzzaman, M. ; Nair, G. Balakrish ; Sack, David A. ; Von Seidlein, Lorenz ; Clemens, John D. ; Song, Manki ; Chun, Jongsik ; Kim, Dong Wook. / Multilocus sequence typing (MLST) analysis of Vibrio cholerae O1 El Tor isolates from Mozambique that harbour the classical CTX prophage. In: Journal of Medical Microbiology. 2006 ; Vol. 55, No. 2. pp. 165-170.
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