Multilocus sequence typing has better discriminatory ability for typing Vibrio cholerae than does pulsed-field gel electrophoresis and provides a measure of phylogenetic relatedness

Mamuka Kotetishvili; O. Colin Stine; Yuansha Chen; Arnold Kreger; Alexander Sulakvelidze; Shanmuga Sozhamannan; J. Glenn Morris

doi:10.1128/JCM.41.5.2191-2196.2003

Multilocus sequence typing has better discriminatory ability for typing Vibrio cholerae than does pulsed-field gel electrophoresis and provides a measure of phylogenetic relatedness

Mamuka Kotetishvili, O. Colin Stine, Yuansha Chen, Arnold Kreger, Alexander Sulakvelidze, Shanmuga Sozhamannan, J. Glenn Morris

Research output: Contribution to journal › Article › peer-review

72 Scopus citations

Abstract

Twenty-two Vibrio cholerae isolates, including some from "epidemic" (O1 and O139) and "nonepidemic" serogroups, were characterized by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) by using three housekeeping genes, gyrB, pgm, and recA; sequence data were also obtained for the virulence-associated genes tcpA, ctxA, and ctxB. Even with the small number of loci used, MLST had better discriminatory ability than did PFGE. On MLST analysis, there was clear clustering of epidemic serogroups; much greater diversity was seen among tcpA- and ctxAB-positive V. cholerae strains from other, nonepidemic serogroups, with a number of tcpA and ctxAB alleles identified.

Original language	English (US)
Pages (from-to)	2191-2196
Number of pages	6
Journal	Journal of Clinical Microbiology
Volume	41
Issue number	5
DOIs	https://doi.org/10.1128/JCM.41.5.2191-2196.2003
State	Published - May 1 2003
Externally published	Yes

ASJC Scopus subject areas

Microbiology (medical)
Microbiology

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10.1128/JCM.41.5.2191-2196.2003

Cite this

Kotetishvili, M., Stine, O. C., Chen, Y., Kreger, A., Sulakvelidze, A., Sozhamannan, S., & Morris, J. G. (2003). Multilocus sequence typing has better discriminatory ability for typing Vibrio cholerae than does pulsed-field gel electrophoresis and provides a measure of phylogenetic relatedness. Journal of Clinical Microbiology, 41(5), 2191-2196. https://doi.org/10.1128/JCM.41.5.2191-2196.2003

Multilocus sequence typing has better discriminatory ability for typing Vibrio cholerae than does pulsed-field gel electrophoresis and provides a measure of phylogenetic relatedness. / Kotetishvili, Mamuka; Stine, O. Colin; Chen, Yuansha et al.
In: Journal of Clinical Microbiology, Vol. 41, No. 5, 01.05.2003, p. 2191-2196.

Research output: Contribution to journal › Article › peer-review

Kotetishvili, M, Stine, OC, Chen, Y, Kreger, A, Sulakvelidze, A, Sozhamannan, S & Morris, JG 2003, 'Multilocus sequence typing has better discriminatory ability for typing Vibrio cholerae than does pulsed-field gel electrophoresis and provides a measure of phylogenetic relatedness', Journal of Clinical Microbiology, vol. 41, no. 5, pp. 2191-2196. https://doi.org/10.1128/JCM.41.5.2191-2196.2003

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AU - Sozhamannan, Shanmuga

AU - Morris, J. Glenn

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Multilocus sequence typing has better discriminatory ability for typing Vibrio cholerae than does pulsed-field gel electrophoresis and provides a measure of phylogenetic relatedness

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