Multilocus sequence typing has better discriminatory ability for typing Vibrio cholerae than does pulsed-field gel electrophoresis and provides a measure of phylogenetic relatedness

Mamuka Kotetishvili, O. Colin Stine, Yuansha Chen, Arnold Kreger, Alexander Sulakvelidze, Shanmuga Sozhamannan, J. Glenn Morris

Research output: Contribution to journalArticlepeer-review

Abstract

Twenty-two Vibrio cholerae isolates, including some from "epidemic" (O1 and O139) and "nonepidemic" serogroups, were characterized by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) by using three housekeeping genes, gyrB, pgm, and recA; sequence data were also obtained for the virulence-associated genes tcpA, ctxA, and ctxB. Even with the small number of loci used, MLST had better discriminatory ability than did PFGE. On MLST analysis, there was clear clustering of epidemic serogroups; much greater diversity was seen among tcpA- and ctxAB-positive V. cholerae strains from other, nonepidemic serogroups, with a number of tcpA and ctxAB alleles identified.

Original languageEnglish (US)
Pages (from-to)2191-2196
Number of pages6
JournalJournal of Clinical Microbiology
Volume41
Issue number5
DOIs
StatePublished - May 1 2003
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

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