Multilineage gene expression precedes commitment in the hemopoietic system

Ming Hu, Diane Krause, Mel Greaves, Saul Sharkis, Michael Dexter, Clare Heyworth, Tariq Enver

Research output: Contribution to journalArticlepeer-review

Abstract

We have tested the hypothesis that multipotential hemopoietic stem and progenitor cells prime several different lineage-affiliated programs of gene activity prior to unilineage commitment and differentiation. Using single cell RT-PCR we show that erythroid (β-globin) and myeloid (myeloperoxidase) gene expression programs can be initiated by the same cell prior to exclusive commitment to the erythroid or granulocytic lineages. Furthermore, the multipotential state is characterized by the coexpression of several lineage- affiliated cytokine receptors. These data support a model of hemopoietic lineage specification in which unilineage commitment is prefaced by a 'promiscuous' phase of multilineage locus activation.

Original languageEnglish (US)
Pages (from-to)774-785
Number of pages12
JournalGenes and Development
Volume11
Issue number6
DOIs
StatePublished - Mar 15 1997

Keywords

  • Hemopoietic stem cells
  • cytokine receptors
  • lineage specification

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

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