Multilineage gene expression precedes commitment in the hemopoietic system

Ming Hu; Diane Krause; Mel Greaves; Saul Sharkis; Michael Dexter; Clare Heyworth; Tariq Enver

doi:10.1101/gad.11.6.774

Multilineage gene expression precedes commitment in the hemopoietic system

Ming Hu, Diane Krause, Mel Greaves, Saul Sharkis, Michael Dexter, Clare Heyworth, Tariq Enver

Research output: Contribution to journal › Article › peer-review

586 Scopus citations

Abstract

We have tested the hypothesis that multipotential hemopoietic stem and progenitor cells prime several different lineage-affiliated programs of gene activity prior to unilineage commitment and differentiation. Using single cell RT-PCR we show that erythroid (β-globin) and myeloid (myeloperoxidase) gene expression programs can be initiated by the same cell prior to exclusive commitment to the erythroid or granulocytic lineages. Furthermore, the multipotential state is characterized by the coexpression of several lineage- affiliated cytokine receptors. These data support a model of hemopoietic lineage specification in which unilineage commitment is prefaced by a 'promiscuous' phase of multilineage locus activation.

Original language	English (US)
Pages (from-to)	774-785
Number of pages	12
Journal	Genes and Development
Volume	11
Issue number	6
DOIs	https://doi.org/10.1101/gad.11.6.774
State	Published - Mar 15 1997
Externally published	Yes

Keywords

Hemopoietic stem cells
cytokine receptors
lineage specification

ASJC Scopus subject areas

Genetics
Developmental Biology

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10.1101/gad.11.6.774

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AU - Hu, Ming

AU - Krause, Diane

AU - Greaves, Mel

AU - Sharkis, Saul

AU - Dexter, Michael

AU - Heyworth, Clare

AU - Enver, Tariq

PY - 1997/3/15

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N2 - We have tested the hypothesis that multipotential hemopoietic stem and progenitor cells prime several different lineage-affiliated programs of gene activity prior to unilineage commitment and differentiation. Using single cell RT-PCR we show that erythroid (β-globin) and myeloid (myeloperoxidase) gene expression programs can be initiated by the same cell prior to exclusive commitment to the erythroid or granulocytic lineages. Furthermore, the multipotential state is characterized by the coexpression of several lineage- affiliated cytokine receptors. These data support a model of hemopoietic lineage specification in which unilineage commitment is prefaced by a 'promiscuous' phase of multilineage locus activation.

AB - We have tested the hypothesis that multipotential hemopoietic stem and progenitor cells prime several different lineage-affiliated programs of gene activity prior to unilineage commitment and differentiation. Using single cell RT-PCR we show that erythroid (β-globin) and myeloid (myeloperoxidase) gene expression programs can be initiated by the same cell prior to exclusive commitment to the erythroid or granulocytic lineages. Furthermore, the multipotential state is characterized by the coexpression of several lineage- affiliated cytokine receptors. These data support a model of hemopoietic lineage specification in which unilineage commitment is prefaced by a 'promiscuous' phase of multilineage locus activation.

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Multilineage gene expression precedes commitment in the hemopoietic system

Abstract

Keywords

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