Multiinstitution study of non-Hodgkin's lymphomas using frozen section immunoperoxidase: The Southeastern Cancer Study Group experience

Michael J Borowitz, S. Newby, R. K. Brynes, J. B. Cousar, C. C. Whitcomb, J. D. Crissman, G. E. Byrne, R. D. Collins

Research output: Contribution to journalArticle

Abstract

This report describes the experience of the Southeastern Cancer Study Group (SECSG) with a transport medium used for immunologic phenotyping of non-Hodgkin's lymphomas. In a 2-mo pilot study, portions of 53 specimens of non-Hodgkin's lymphoma from 4 member institutions of the SECSG and affiliated community hospitals were sent by regular mail to a central laboratory. Immunologic phenotyping was carried out using a frozen section immunoperoxidase technique. In 48 of the cases, a clear-cut immunologic phenotype was obtained. Thirty-four tumors were of B cell origin and 7 had T cell markers. Six of the remaining lymphomas had neither B nor T cell markers, and the 7th had both. In 12 cases, phenotyping was also carried out at the originating institution using conventional cell suspension techniques; agreement between the 2 methods was excellent. The immunologic results were correlated with histopathologic diagnosis standardized using the Working Formulation for non-Hodgkin's lymphomas. It was found that the low grade tumors were all B cell, but that the intermediate grade tumors were very heterogeneous immunologically. About one-fourth of the diffuse, intermediate grade or miscellaneous tumors had T cell markers. Our results indicate that immunologic phenotyping may be performed satisfactorily on transported material, making multiinstitution studies on the prognostic significance of immunologic phenotype in non-Hodgkin's lymphomas feasible.

Original languageEnglish (US)
Pages (from-to)1147-1152
Number of pages6
JournalBlood
Volume63
Issue number5
StatePublished - 1984
Externally publishedYes

Fingerprint

Frozen Sections
Non-Hodgkin's Lymphoma
T-cells
Tumors
Neoplasms
Cells
T-Lymphocytes
B-Lymphocytes
Phenotype
Suspensions
Community Hospital
Postal Service
Immunoenzyme Techniques
Lymphoma

ASJC Scopus subject areas

  • Hematology

Cite this

Borowitz, M. J., Newby, S., Brynes, R. K., Cousar, J. B., Whitcomb, C. C., Crissman, J. D., ... Collins, R. D. (1984). Multiinstitution study of non-Hodgkin's lymphomas using frozen section immunoperoxidase: The Southeastern Cancer Study Group experience. Blood, 63(5), 1147-1152.

Multiinstitution study of non-Hodgkin's lymphomas using frozen section immunoperoxidase : The Southeastern Cancer Study Group experience. / Borowitz, Michael J; Newby, S.; Brynes, R. K.; Cousar, J. B.; Whitcomb, C. C.; Crissman, J. D.; Byrne, G. E.; Collins, R. D.

In: Blood, Vol. 63, No. 5, 1984, p. 1147-1152.

Research output: Contribution to journalArticle

Borowitz, MJ, Newby, S, Brynes, RK, Cousar, JB, Whitcomb, CC, Crissman, JD, Byrne, GE & Collins, RD 1984, 'Multiinstitution study of non-Hodgkin's lymphomas using frozen section immunoperoxidase: The Southeastern Cancer Study Group experience', Blood, vol. 63, no. 5, pp. 1147-1152.
Borowitz, Michael J ; Newby, S. ; Brynes, R. K. ; Cousar, J. B. ; Whitcomb, C. C. ; Crissman, J. D. ; Byrne, G. E. ; Collins, R. D. / Multiinstitution study of non-Hodgkin's lymphomas using frozen section immunoperoxidase : The Southeastern Cancer Study Group experience. In: Blood. 1984 ; Vol. 63, No. 5. pp. 1147-1152.
@article{fe17ae0cd8d9487a8670c58a73ae9a67,
title = "Multiinstitution study of non-Hodgkin's lymphomas using frozen section immunoperoxidase: The Southeastern Cancer Study Group experience",
abstract = "This report describes the experience of the Southeastern Cancer Study Group (SECSG) with a transport medium used for immunologic phenotyping of non-Hodgkin's lymphomas. In a 2-mo pilot study, portions of 53 specimens of non-Hodgkin's lymphoma from 4 member institutions of the SECSG and affiliated community hospitals were sent by regular mail to a central laboratory. Immunologic phenotyping was carried out using a frozen section immunoperoxidase technique. In 48 of the cases, a clear-cut immunologic phenotype was obtained. Thirty-four tumors were of B cell origin and 7 had T cell markers. Six of the remaining lymphomas had neither B nor T cell markers, and the 7th had both. In 12 cases, phenotyping was also carried out at the originating institution using conventional cell suspension techniques; agreement between the 2 methods was excellent. The immunologic results were correlated with histopathologic diagnosis standardized using the Working Formulation for non-Hodgkin's lymphomas. It was found that the low grade tumors were all B cell, but that the intermediate grade tumors were very heterogeneous immunologically. About one-fourth of the diffuse, intermediate grade or miscellaneous tumors had T cell markers. Our results indicate that immunologic phenotyping may be performed satisfactorily on transported material, making multiinstitution studies on the prognostic significance of immunologic phenotype in non-Hodgkin's lymphomas feasible.",
author = "Borowitz, {Michael J} and S. Newby and Brynes, {R. K.} and Cousar, {J. B.} and Whitcomb, {C. C.} and Crissman, {J. D.} and Byrne, {G. E.} and Collins, {R. D.}",
year = "1984",
language = "English (US)",
volume = "63",
pages = "1147--1152",
journal = "Blood",
issn = "0006-4971",
publisher = "American Society of Hematology",
number = "5",

}

TY - JOUR

T1 - Multiinstitution study of non-Hodgkin's lymphomas using frozen section immunoperoxidase

T2 - The Southeastern Cancer Study Group experience

AU - Borowitz, Michael J

AU - Newby, S.

AU - Brynes, R. K.

AU - Cousar, J. B.

AU - Whitcomb, C. C.

AU - Crissman, J. D.

AU - Byrne, G. E.

AU - Collins, R. D.

PY - 1984

Y1 - 1984

N2 - This report describes the experience of the Southeastern Cancer Study Group (SECSG) with a transport medium used for immunologic phenotyping of non-Hodgkin's lymphomas. In a 2-mo pilot study, portions of 53 specimens of non-Hodgkin's lymphoma from 4 member institutions of the SECSG and affiliated community hospitals were sent by regular mail to a central laboratory. Immunologic phenotyping was carried out using a frozen section immunoperoxidase technique. In 48 of the cases, a clear-cut immunologic phenotype was obtained. Thirty-four tumors were of B cell origin and 7 had T cell markers. Six of the remaining lymphomas had neither B nor T cell markers, and the 7th had both. In 12 cases, phenotyping was also carried out at the originating institution using conventional cell suspension techniques; agreement between the 2 methods was excellent. The immunologic results were correlated with histopathologic diagnosis standardized using the Working Formulation for non-Hodgkin's lymphomas. It was found that the low grade tumors were all B cell, but that the intermediate grade tumors were very heterogeneous immunologically. About one-fourth of the diffuse, intermediate grade or miscellaneous tumors had T cell markers. Our results indicate that immunologic phenotyping may be performed satisfactorily on transported material, making multiinstitution studies on the prognostic significance of immunologic phenotype in non-Hodgkin's lymphomas feasible.

AB - This report describes the experience of the Southeastern Cancer Study Group (SECSG) with a transport medium used for immunologic phenotyping of non-Hodgkin's lymphomas. In a 2-mo pilot study, portions of 53 specimens of non-Hodgkin's lymphoma from 4 member institutions of the SECSG and affiliated community hospitals were sent by regular mail to a central laboratory. Immunologic phenotyping was carried out using a frozen section immunoperoxidase technique. In 48 of the cases, a clear-cut immunologic phenotype was obtained. Thirty-four tumors were of B cell origin and 7 had T cell markers. Six of the remaining lymphomas had neither B nor T cell markers, and the 7th had both. In 12 cases, phenotyping was also carried out at the originating institution using conventional cell suspension techniques; agreement between the 2 methods was excellent. The immunologic results were correlated with histopathologic diagnosis standardized using the Working Formulation for non-Hodgkin's lymphomas. It was found that the low grade tumors were all B cell, but that the intermediate grade tumors were very heterogeneous immunologically. About one-fourth of the diffuse, intermediate grade or miscellaneous tumors had T cell markers. Our results indicate that immunologic phenotyping may be performed satisfactorily on transported material, making multiinstitution studies on the prognostic significance of immunologic phenotype in non-Hodgkin's lymphomas feasible.

UR - http://www.scopus.com/inward/record.url?scp=0021267935&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021267935&partnerID=8YFLogxK

M3 - Article

C2 - 6370333

AN - SCOPUS:0021267935

VL - 63

SP - 1147

EP - 1152

JO - Blood

JF - Blood

SN - 0006-4971

IS - 5

ER -