Multifunctional aliphatic polyester nanofibers for tissue engineering.

Jianan Zhan, Anirudha Singh, Zhe Zhang, Ling Huang, Jennifer Hartt Elisseeff

Research output: Contribution to journalArticle

Abstract

Electrospun fibers based on aliphatic polyesters, such as poly(ε-caprolactone) (PCL), have been widely used in regenerative medicine and drug delivery applications due to their biocompatibility, low cost and ease of fabrication. However, these aliphatic polyester fibers are hydrophobic in nature, resulting in poor wettability, and they lack functional groups for decorating the scaffold with chemical and biological cues. Current strategies employed to overcome these challenges include coating and blending the fibers with bioactive components or chemically modifying the fibers with plasma treatment and reactants. In the present study, we report on designing multifunctional electrospun nanofibers based on the inclusion complex of PCL-α-cyclodextrin (PCL-α-CD), which provides both structural support and multiple functionalities for further conjugation of bioactive components. This strategy is independent of any chemical modification of the PCL main chain, and electrospinning of PCL-α-CD is as easy as electrospinning PCL. Here, we describe synthesis of the PCL-α-CD electrospun nanofibers, elucidate composition and structure, and demonstrate the utility of functional groups on the fibers by conjugating a fluorescent small molecule and a polymeric-nanobead to the nanofibers. Furthermore, we demonstrate the application of PCL-α-CD nanofibers for promoting osteogenic differentiation of human adipose-derived stem cells (hADSCs), which induced a higher level of expression of osteogenic markers and enhanced production of extracellular matrix (ECM) proteins or molecules compared with control PCL fibers.

Original languageEnglish (US)
Pages (from-to)202-212
Number of pages11
JournalBiomatter
Volume2
Issue number4
StatePublished - Oct 2012
Externally publishedYes

Fingerprint

Nanofibers
Polyesters
Cyclodextrins
Tissue Engineering
Tissue engineering
Fibers
Electrospinning
Wettability
Functional groups
Regenerative Medicine
Extracellular Matrix Proteins
Cues
Molecules
Stem Cells
Chemical modification
Stem cells
Drug delivery
Biocompatibility
Costs and Cost Analysis
Scaffolds

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Medicine (miscellaneous)
  • Medicine(all)

Cite this

Multifunctional aliphatic polyester nanofibers for tissue engineering. / Zhan, Jianan; Singh, Anirudha; Zhang, Zhe; Huang, Ling; Elisseeff, Jennifer Hartt.

In: Biomatter, Vol. 2, No. 4, 10.2012, p. 202-212.

Research output: Contribution to journalArticle

Zhan, Jianan ; Singh, Anirudha ; Zhang, Zhe ; Huang, Ling ; Elisseeff, Jennifer Hartt. / Multifunctional aliphatic polyester nanofibers for tissue engineering. In: Biomatter. 2012 ; Vol. 2, No. 4. pp. 202-212.
@article{b7227e7097a84308893b4fa0e8f80497,
title = "Multifunctional aliphatic polyester nanofibers for tissue engineering.",
abstract = "Electrospun fibers based on aliphatic polyesters, such as poly(ε-caprolactone) (PCL), have been widely used in regenerative medicine and drug delivery applications due to their biocompatibility, low cost and ease of fabrication. However, these aliphatic polyester fibers are hydrophobic in nature, resulting in poor wettability, and they lack functional groups for decorating the scaffold with chemical and biological cues. Current strategies employed to overcome these challenges include coating and blending the fibers with bioactive components or chemically modifying the fibers with plasma treatment and reactants. In the present study, we report on designing multifunctional electrospun nanofibers based on the inclusion complex of PCL-α-cyclodextrin (PCL-α-CD), which provides both structural support and multiple functionalities for further conjugation of bioactive components. This strategy is independent of any chemical modification of the PCL main chain, and electrospinning of PCL-α-CD is as easy as electrospinning PCL. Here, we describe synthesis of the PCL-α-CD electrospun nanofibers, elucidate composition and structure, and demonstrate the utility of functional groups on the fibers by conjugating a fluorescent small molecule and a polymeric-nanobead to the nanofibers. Furthermore, we demonstrate the application of PCL-α-CD nanofibers for promoting osteogenic differentiation of human adipose-derived stem cells (hADSCs), which induced a higher level of expression of osteogenic markers and enhanced production of extracellular matrix (ECM) proteins or molecules compared with control PCL fibers.",
author = "Jianan Zhan and Anirudha Singh and Zhe Zhang and Ling Huang and Elisseeff, {Jennifer Hartt}",
year = "2012",
month = "10",
language = "English (US)",
volume = "2",
pages = "202--212",
journal = "Biomatter",
issn = "2159-2527",
publisher = "Landes Bioscience",
number = "4",

}

TY - JOUR

T1 - Multifunctional aliphatic polyester nanofibers for tissue engineering.

AU - Zhan, Jianan

AU - Singh, Anirudha

AU - Zhang, Zhe

AU - Huang, Ling

AU - Elisseeff, Jennifer Hartt

PY - 2012/10

Y1 - 2012/10

N2 - Electrospun fibers based on aliphatic polyesters, such as poly(ε-caprolactone) (PCL), have been widely used in regenerative medicine and drug delivery applications due to their biocompatibility, low cost and ease of fabrication. However, these aliphatic polyester fibers are hydrophobic in nature, resulting in poor wettability, and they lack functional groups for decorating the scaffold with chemical and biological cues. Current strategies employed to overcome these challenges include coating and blending the fibers with bioactive components or chemically modifying the fibers with plasma treatment and reactants. In the present study, we report on designing multifunctional electrospun nanofibers based on the inclusion complex of PCL-α-cyclodextrin (PCL-α-CD), which provides both structural support and multiple functionalities for further conjugation of bioactive components. This strategy is independent of any chemical modification of the PCL main chain, and electrospinning of PCL-α-CD is as easy as electrospinning PCL. Here, we describe synthesis of the PCL-α-CD electrospun nanofibers, elucidate composition and structure, and demonstrate the utility of functional groups on the fibers by conjugating a fluorescent small molecule and a polymeric-nanobead to the nanofibers. Furthermore, we demonstrate the application of PCL-α-CD nanofibers for promoting osteogenic differentiation of human adipose-derived stem cells (hADSCs), which induced a higher level of expression of osteogenic markers and enhanced production of extracellular matrix (ECM) proteins or molecules compared with control PCL fibers.

AB - Electrospun fibers based on aliphatic polyesters, such as poly(ε-caprolactone) (PCL), have been widely used in regenerative medicine and drug delivery applications due to their biocompatibility, low cost and ease of fabrication. However, these aliphatic polyester fibers are hydrophobic in nature, resulting in poor wettability, and they lack functional groups for decorating the scaffold with chemical and biological cues. Current strategies employed to overcome these challenges include coating and blending the fibers with bioactive components or chemically modifying the fibers with plasma treatment and reactants. In the present study, we report on designing multifunctional electrospun nanofibers based on the inclusion complex of PCL-α-cyclodextrin (PCL-α-CD), which provides both structural support and multiple functionalities for further conjugation of bioactive components. This strategy is independent of any chemical modification of the PCL main chain, and electrospinning of PCL-α-CD is as easy as electrospinning PCL. Here, we describe synthesis of the PCL-α-CD electrospun nanofibers, elucidate composition and structure, and demonstrate the utility of functional groups on the fibers by conjugating a fluorescent small molecule and a polymeric-nanobead to the nanofibers. Furthermore, we demonstrate the application of PCL-α-CD nanofibers for promoting osteogenic differentiation of human adipose-derived stem cells (hADSCs), which induced a higher level of expression of osteogenic markers and enhanced production of extracellular matrix (ECM) proteins or molecules compared with control PCL fibers.

UR - http://www.scopus.com/inward/record.url?scp=84885345249&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84885345249&partnerID=8YFLogxK

M3 - Article

C2 - 23507886

AN - SCOPUS:84885345249

VL - 2

SP - 202

EP - 212

JO - Biomatter

JF - Biomatter

SN - 2159-2527

IS - 4

ER -