Multicolor spectral karyotyping of human chromosomes

E. Schröck; S. Du Manoir; T. Veldman; B. Schoell; J. Wienberg; M. A. Ferguson-Smith; Y. Ning; D. H. Ledbetter; I. Bar-Am; D. Soenksen; Y. Garini; T. Ried

doi:10.1126/science.273.5274.494

Multicolor spectral karyotyping of human chromosomes

E. Schröck, S. Du Manoir, T. Veldman, B. Schoell, J. Wienberg, M. A. Ferguson-Smith, Y. Ning, D. H. Ledbetter, I. Bar-Am, D. Soenksen, Y. Garini, T. Ried

Research output: Contribution to journal › Article › peer-review

1423 Scopus citations

Abstract

The simultaneous and unequivocal discernment of all human chromosomes in different colors would be of significant clinical and biologic importance. Whole-genome scanning by spectral karyotyping allowed instantaneous visualization of defined emission spectra for each human chromosome after fluorescence in situ hybridization. By means of computer separation (classification) of spectra, spectrally overlapping chromosome-specific DNA probes could be resolved, and all human chromosomes were simultaneously identified.

Original language	English (US)
Pages (from-to)	494-497
Number of pages	4
Journal	Science
Volume	273
Issue number	5274
DOIs	https://doi.org/10.1126/science.273.5274.494
State	Published - Jul 26 1996
Externally published	Yes

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10.1126/science.273.5274.494

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title = "Multicolor spectral karyotyping of human chromosomes",

abstract = "The simultaneous and unequivocal discernment of all human chromosomes in different colors would be of significant clinical and biologic importance. Whole-genome scanning by spectral karyotyping allowed instantaneous visualization of defined emission spectra for each human chromosome after fluorescence in situ hybridization. By means of computer separation (classification) of spectra, spectrally overlapping chromosome-specific DNA probes could be resolved, and all human chromosomes were simultaneously identified.",

author = "E. Schr{\"o}ck and {Du Manoir}, S. and T. Veldman and B. Schoell and J. Wienberg and Ferguson-Smith, {M. A.} and Y. Ning and Ledbetter, {D. H.} and I. Bar-Am and D. Soenksen and Y. Garini and T. Ried",

year = "1996",

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doi = "10.1126/science.273.5274.494",

language = "English (US)",

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T1 - Multicolor spectral karyotyping of human chromosomes

AU - Schröck, E.

AU - Du Manoir, S.

AU - Veldman, T.

AU - Schoell, B.

AU - Wienberg, J.

AU - Ferguson-Smith, M. A.

AU - Ning, Y.

AU - Ledbetter, D. H.

AU - Bar-Am, I.

AU - Soenksen, D.

AU - Garini, Y.

AU - Ried, T.

PY - 1996/7/26

Y1 - 1996/7/26

N2 - The simultaneous and unequivocal discernment of all human chromosomes in different colors would be of significant clinical and biologic importance. Whole-genome scanning by spectral karyotyping allowed instantaneous visualization of defined emission spectra for each human chromosome after fluorescence in situ hybridization. By means of computer separation (classification) of spectra, spectrally overlapping chromosome-specific DNA probes could be resolved, and all human chromosomes were simultaneously identified.

AB - The simultaneous and unequivocal discernment of all human chromosomes in different colors would be of significant clinical and biologic importance. Whole-genome scanning by spectral karyotyping allowed instantaneous visualization of defined emission spectra for each human chromosome after fluorescence in situ hybridization. By means of computer separation (classification) of spectra, spectrally overlapping chromosome-specific DNA probes could be resolved, and all human chromosomes were simultaneously identified.

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EP - 497

JO - Science

JF - Science

IS - 5274

ER -

Multicolor spectral karyotyping of human chromosomes

Abstract

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