Multicenter Study of Epidemiological Cutoff Values and Detection of Resistance in Candida spp. to Anidulafungin, Caspofungin, and Micafungin Using the Sensititre YeastOne Colorimetric Method

A. Espinel-Ingroff, M. Alvarez-Fernandez, E. Cantón, P. L. Carver, S. C A Chen, G. Eschenauer, D. L. Getsinger, G. M. Gonzalez, N. P. Govender, A. Grancini, K. E. Hanson, S. E. Kidd, K. Klinker, C. J. Kubin, J. V. Kus, S. R. Lockhart, J. Meletiadis, A. J. Morris, T. Pelaez, G. Quindós & 24 others M. Rodriguez-Iglesias, F. Sánchez-Reus, Shmuel Shoham, N. L. Wengenack, N. Borrell Solé, J. Echeverria, J. Esperalba, E. Gómez G De La Pedrosa, I. García García, M. J. Linares, F. Marco, P. Merino, J. Pemán, L. Pérez Del Molino, E. Roselló Mayans, C. Rubio Calvo, M. Ruiz Pérez De Pipaon, G. Yagüe, G. Garcia-Effron, J. Guinea, D. S. Perlin, M. Sanguinetti, R. Shields, J. Turnidge

Research output: Contribution to journalArticle

Abstract

Neither breakpoints (BPs) nor epidemiological cutoff values (ECVs) have been established for Candida spp. with anidulafungin, caspofungin, and micafungin when using the Sensititre YeastOne (SYO) broth dilution colorimetric method. In addition, reference caspofungin MICs have so far proven to be unreliable. Candida species wild-type (WT) MIC distributions (for microorganisms in a species/drug combination with no detectable phenotypic resistance) were established for 6,007 Candida albicans, 186 C. dubliniensis, 3,188 C. glabrata complex, 119 C. guilliermondii, 493 C. krusei, 205 C. lusitaniae, 3,136 C. parapsilosis complex, and 1,016 C. tropicalis isolates. SYO MIC data gathered from 38 laboratories in Australia, Canada, Europe, Mexico, New Zealand, South Africa, and the United States were pooled to statistically define SYO ECVs. ECVs for anidulafungin, caspofungin, and micafungin encompassing >97.5% of the statistically modeled population were, respectively, 0.12,0.25, and 0.06 μg/ml for C. albicans, 0.12, 0.25, and 0.03 μg/ml for C. glabrata complex, 4, 2, and 4 μg/ml for C. parapsilosis complex, 0.5,0.25, and 0.06 μg/ml for C. tropicalis, 0.25,1, and 0.25 μg/ml for C. krusei, 0.25,1, and 0.12 μg/ml for C. lusitaniae, 4,2, and 2 μg/ml for C. guilliermondii, and 0.25,0.25, and 0.12 μg/ml for C. dubliniensis. Species-specific SYO ECVs for anidulafungin, caspofungin, and micafungin correctly classified 72 (88.9%), 74 (91.4%), 76 (93.8%), respectively, of 81 Candida isolates with identified fks mutations. SYO ECVs may aid in detecting non-WT isolates with reduced susceptibility to anidulafungin, micafungin, and especially caspofungin, since testing the susceptibilities of Candida spp. to caspofungin by reference methodologies is not recommended.

Original languageEnglish (US)
Pages (from-to)6725-6732
Number of pages8
JournalAntimicrobial Agents and Chemotherapy
Volume59
Issue number11
DOIs
StatePublished - Nov 1 2015

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caspofungin
anidulafungin
Candida
Multicenter Studies
Candida albicans
Drug Combinations
South Africa
Mexico
New Zealand
Canada
micafungin
Mutation

ASJC Scopus subject areas

  • Pharmacology (medical)
  • Pharmacology
  • Infectious Diseases

Cite this

Multicenter Study of Epidemiological Cutoff Values and Detection of Resistance in Candida spp. to Anidulafungin, Caspofungin, and Micafungin Using the Sensititre YeastOne Colorimetric Method. / Espinel-Ingroff, A.; Alvarez-Fernandez, M.; Cantón, E.; Carver, P. L.; Chen, S. C A; Eschenauer, G.; Getsinger, D. L.; Gonzalez, G. M.; Govender, N. P.; Grancini, A.; Hanson, K. E.; Kidd, S. E.; Klinker, K.; Kubin, C. J.; Kus, J. V.; Lockhart, S. R.; Meletiadis, J.; Morris, A. J.; Pelaez, T.; Quindós, G.; Rodriguez-Iglesias, M.; Sánchez-Reus, F.; Shoham, Shmuel; Wengenack, N. L.; Borrell Solé, N.; Echeverria, J.; Esperalba, J.; De La Pedrosa, E. Gómez G; García García, I.; Linares, M. J.; Marco, F.; Merino, P.; Pemán, J.; Pérez Del Molino, L.; Roselló Mayans, E.; Rubio Calvo, C.; Ruiz Pérez De Pipaon, M.; Yagüe, G.; Garcia-Effron, G.; Guinea, J.; Perlin, D. S.; Sanguinetti, M.; Shields, R.; Turnidge, J.

In: Antimicrobial Agents and Chemotherapy, Vol. 59, No. 11, 01.11.2015, p. 6725-6732.

Research output: Contribution to journalArticle

Espinel-Ingroff, A, Alvarez-Fernandez, M, Cantón, E, Carver, PL, Chen, SCA, Eschenauer, G, Getsinger, DL, Gonzalez, GM, Govender, NP, Grancini, A, Hanson, KE, Kidd, SE, Klinker, K, Kubin, CJ, Kus, JV, Lockhart, SR, Meletiadis, J, Morris, AJ, Pelaez, T, Quindós, G, Rodriguez-Iglesias, M, Sánchez-Reus, F, Shoham, S, Wengenack, NL, Borrell Solé, N, Echeverria, J, Esperalba, J, De La Pedrosa, EGG, García García, I, Linares, MJ, Marco, F, Merino, P, Pemán, J, Pérez Del Molino, L, Roselló Mayans, E, Rubio Calvo, C, Ruiz Pérez De Pipaon, M, Yagüe, G, Garcia-Effron, G, Guinea, J, Perlin, DS, Sanguinetti, M, Shields, R & Turnidge, J 2015, 'Multicenter Study of Epidemiological Cutoff Values and Detection of Resistance in Candida spp. to Anidulafungin, Caspofungin, and Micafungin Using the Sensititre YeastOne Colorimetric Method', Antimicrobial Agents and Chemotherapy, vol. 59, no. 11, pp. 6725-6732. https://doi.org/10.1128/AAC.01250-15
Espinel-Ingroff, A. ; Alvarez-Fernandez, M. ; Cantón, E. ; Carver, P. L. ; Chen, S. C A ; Eschenauer, G. ; Getsinger, D. L. ; Gonzalez, G. M. ; Govender, N. P. ; Grancini, A. ; Hanson, K. E. ; Kidd, S. E. ; Klinker, K. ; Kubin, C. J. ; Kus, J. V. ; Lockhart, S. R. ; Meletiadis, J. ; Morris, A. J. ; Pelaez, T. ; Quindós, G. ; Rodriguez-Iglesias, M. ; Sánchez-Reus, F. ; Shoham, Shmuel ; Wengenack, N. L. ; Borrell Solé, N. ; Echeverria, J. ; Esperalba, J. ; De La Pedrosa, E. Gómez G ; García García, I. ; Linares, M. J. ; Marco, F. ; Merino, P. ; Pemán, J. ; Pérez Del Molino, L. ; Roselló Mayans, E. ; Rubio Calvo, C. ; Ruiz Pérez De Pipaon, M. ; Yagüe, G. ; Garcia-Effron, G. ; Guinea, J. ; Perlin, D. S. ; Sanguinetti, M. ; Shields, R. ; Turnidge, J. / Multicenter Study of Epidemiological Cutoff Values and Detection of Resistance in Candida spp. to Anidulafungin, Caspofungin, and Micafungin Using the Sensititre YeastOne Colorimetric Method. In: Antimicrobial Agents and Chemotherapy. 2015 ; Vol. 59, No. 11. pp. 6725-6732.
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title = "Multicenter Study of Epidemiological Cutoff Values and Detection of Resistance in Candida spp. to Anidulafungin, Caspofungin, and Micafungin Using the Sensititre YeastOne Colorimetric Method",
abstract = "Neither breakpoints (BPs) nor epidemiological cutoff values (ECVs) have been established for Candida spp. with anidulafungin, caspofungin, and micafungin when using the Sensititre YeastOne (SYO) broth dilution colorimetric method. In addition, reference caspofungin MICs have so far proven to be unreliable. Candida species wild-type (WT) MIC distributions (for microorganisms in a species/drug combination with no detectable phenotypic resistance) were established for 6,007 Candida albicans, 186 C. dubliniensis, 3,188 C. glabrata complex, 119 C. guilliermondii, 493 C. krusei, 205 C. lusitaniae, 3,136 C. parapsilosis complex, and 1,016 C. tropicalis isolates. SYO MIC data gathered from 38 laboratories in Australia, Canada, Europe, Mexico, New Zealand, South Africa, and the United States were pooled to statistically define SYO ECVs. ECVs for anidulafungin, caspofungin, and micafungin encompassing >97.5{\%} of the statistically modeled population were, respectively, 0.12,0.25, and 0.06 μg/ml for C. albicans, 0.12, 0.25, and 0.03 μg/ml for C. glabrata complex, 4, 2, and 4 μg/ml for C. parapsilosis complex, 0.5,0.25, and 0.06 μg/ml for C. tropicalis, 0.25,1, and 0.25 μg/ml for C. krusei, 0.25,1, and 0.12 μg/ml for C. lusitaniae, 4,2, and 2 μg/ml for C. guilliermondii, and 0.25,0.25, and 0.12 μg/ml for C. dubliniensis. Species-specific SYO ECVs for anidulafungin, caspofungin, and micafungin correctly classified 72 (88.9{\%}), 74 (91.4{\%}), 76 (93.8{\%}), respectively, of 81 Candida isolates with identified fks mutations. SYO ECVs may aid in detecting non-WT isolates with reduced susceptibility to anidulafungin, micafungin, and especially caspofungin, since testing the susceptibilities of Candida spp. to caspofungin by reference methodologies is not recommended.",
author = "A. Espinel-Ingroff and M. Alvarez-Fernandez and E. Cant{\'o}n and Carver, {P. L.} and Chen, {S. C A} and G. Eschenauer and Getsinger, {D. L.} and Gonzalez, {G. M.} and Govender, {N. P.} and A. Grancini and Hanson, {K. E.} and Kidd, {S. E.} and K. Klinker and Kubin, {C. J.} and Kus, {J. V.} and Lockhart, {S. R.} and J. Meletiadis and Morris, {A. J.} and T. Pelaez and G. Quind{\'o}s and M. Rodriguez-Iglesias and F. S{\'a}nchez-Reus and Shmuel Shoham and Wengenack, {N. L.} and {Borrell Sol{\'e}}, N. and J. Echeverria and J. Esperalba and {De La Pedrosa}, {E. G{\'o}mez G} and {Garc{\'i}a Garc{\'i}a}, I. and Linares, {M. J.} and F. Marco and P. Merino and J. Pem{\'a}n and {P{\'e}rez Del Molino}, L. and {Rosell{\'o} Mayans}, E. and {Rubio Calvo}, C. and {Ruiz P{\'e}rez De Pipaon}, M. and G. Yag{\"u}e and G. Garcia-Effron and J. Guinea and Perlin, {D. S.} and M. Sanguinetti and R. Shields and J. Turnidge",
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TY - JOUR

T1 - Multicenter Study of Epidemiological Cutoff Values and Detection of Resistance in Candida spp. to Anidulafungin, Caspofungin, and Micafungin Using the Sensititre YeastOne Colorimetric Method

AU - Espinel-Ingroff, A.

AU - Alvarez-Fernandez, M.

AU - Cantón, E.

AU - Carver, P. L.

AU - Chen, S. C A

AU - Eschenauer, G.

AU - Getsinger, D. L.

AU - Gonzalez, G. M.

AU - Govender, N. P.

AU - Grancini, A.

AU - Hanson, K. E.

AU - Kidd, S. E.

AU - Klinker, K.

AU - Kubin, C. J.

AU - Kus, J. V.

AU - Lockhart, S. R.

AU - Meletiadis, J.

AU - Morris, A. J.

AU - Pelaez, T.

AU - Quindós, G.

AU - Rodriguez-Iglesias, M.

AU - Sánchez-Reus, F.

AU - Shoham, Shmuel

AU - Wengenack, N. L.

AU - Borrell Solé, N.

AU - Echeverria, J.

AU - Esperalba, J.

AU - De La Pedrosa, E. Gómez G

AU - García García, I.

AU - Linares, M. J.

AU - Marco, F.

AU - Merino, P.

AU - Pemán, J.

AU - Pérez Del Molino, L.

AU - Roselló Mayans, E.

AU - Rubio Calvo, C.

AU - Ruiz Pérez De Pipaon, M.

AU - Yagüe, G.

AU - Garcia-Effron, G.

AU - Guinea, J.

AU - Perlin, D. S.

AU - Sanguinetti, M.

AU - Shields, R.

AU - Turnidge, J.

PY - 2015/11/1

Y1 - 2015/11/1

N2 - Neither breakpoints (BPs) nor epidemiological cutoff values (ECVs) have been established for Candida spp. with anidulafungin, caspofungin, and micafungin when using the Sensititre YeastOne (SYO) broth dilution colorimetric method. In addition, reference caspofungin MICs have so far proven to be unreliable. Candida species wild-type (WT) MIC distributions (for microorganisms in a species/drug combination with no detectable phenotypic resistance) were established for 6,007 Candida albicans, 186 C. dubliniensis, 3,188 C. glabrata complex, 119 C. guilliermondii, 493 C. krusei, 205 C. lusitaniae, 3,136 C. parapsilosis complex, and 1,016 C. tropicalis isolates. SYO MIC data gathered from 38 laboratories in Australia, Canada, Europe, Mexico, New Zealand, South Africa, and the United States were pooled to statistically define SYO ECVs. ECVs for anidulafungin, caspofungin, and micafungin encompassing >97.5% of the statistically modeled population were, respectively, 0.12,0.25, and 0.06 μg/ml for C. albicans, 0.12, 0.25, and 0.03 μg/ml for C. glabrata complex, 4, 2, and 4 μg/ml for C. parapsilosis complex, 0.5,0.25, and 0.06 μg/ml for C. tropicalis, 0.25,1, and 0.25 μg/ml for C. krusei, 0.25,1, and 0.12 μg/ml for C. lusitaniae, 4,2, and 2 μg/ml for C. guilliermondii, and 0.25,0.25, and 0.12 μg/ml for C. dubliniensis. Species-specific SYO ECVs for anidulafungin, caspofungin, and micafungin correctly classified 72 (88.9%), 74 (91.4%), 76 (93.8%), respectively, of 81 Candida isolates with identified fks mutations. SYO ECVs may aid in detecting non-WT isolates with reduced susceptibility to anidulafungin, micafungin, and especially caspofungin, since testing the susceptibilities of Candida spp. to caspofungin by reference methodologies is not recommended.

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